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首页> 外文期刊>Environmental research >Tetracycline affects the toxicity of P25 n-TiO_2 towards marine microalgae Chlorella sp
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Tetracycline affects the toxicity of P25 n-TiO_2 towards marine microalgae Chlorella sp

机译:四环素会影响P25 N-TiO_2对海洋微藻Chortella sp的毒性

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Pollutants such as n-TiO_2 and tetracycline enter the marine environment through various sources starting from their production until disposal. Hence, it is vital to determine the interactive effect of one pollutant with the other when they coexist in the environment. In the present study, the effect of antibiotic - tetracycline (TC) on the toxicity of P25 n-TiO_2 was studied with marine microalgae, Chlorella sp. The impact of TC (1 mg L~(-1)) on five different concentrations of n-TiO_2 (0.25, 0.5, 1, 2 and 4 mg L~(-1)) under both visible and UV-A illuminations was evaluated. Effective diameter of n-TiO_2 in ASW at 0th h increased from 690.69 ± 19.55 nm (0.25 mg L~(-1)) to 1183.04 ± 37.10nm (0.25mgL~(-1) + 1 mg L~(-1)) and 971.51 ± 14.61 nm (4mgL~(-1)) to 1324.12 ± 11.59nm (4mgL~(-1) + 1 mg L~(-1)) in presence of TC. A significant increase in the toxicity of 4mgL~(-1) n-TiO_2 upon the addition of TC (68.16 ± 0.37% under visible and 80.21 ± 0.3% under UV-A condition) was observed. No significant difference in toxicity was observed between visible and UV-A illuminations. Further the toxicity data was corroborated through the measurement of oxidative stress and antioxidant enzyme activities. Independent action model showed antagonistic effect for lower concentrations of n-TiO_2 and additive effect for higher concentrations of n-TiO_2 when present in mixture with TC under both illuminations. For the higher mixture concentration of 4 mgL~(-1) n-TiO_2 and 1 mg L~(-1) TC, the percentage TC removal was about 55.29% and 30% and the corresponding TOC removal was found to be 54.29% and 31.04% under visible and UV-A illuminations respectively. The site of ROS generation in Chlorella sp. was identified with electron transfer chain inhibitors. Both mitochondria and chloroplast acted as the site for the ROS generation in Chlorella sp. The SEM images of the algal cells upon exposure to n-TiO_2 and mixture revealed the aggregation of cells and distortion of cell membrane.
机译:污染物如N-TiO_2和四环素通过从其生产开始的各种来源进入海洋环境,直到处置。因此,当他们在环境中共存时,对另一个污染物的交互式效果至关重要。在本研究中,用海洋微藻,小球藻SP研究了抗生素 - 四环素(TC)对P25 N-TiO_2的毒性的影响。评估了在可见和UV-A照明下的五种不同浓度的N-TiO_2(0.25,1,2和4mg L〜(-1)的五种不同浓度的Tc(1mg L〜(-1)) 。 0h中ASW的N-TiO_2的有效直径从690.69±19.55 nm(0.25mg l〜(-1))增加到1183.04±37.10nm(0.25mgl〜(-1)+ 1 mg l〜(1))在TC存在下,971.51±14.61nm(4mgl〜(-1(-1))至1324.12±11.59nm(4mgl〜(-1)+ 1mg l〜(-1))。观察到在添加TC时4mgL〜(-1)n-TiO_2的毒性显着增加(在可见的可见下的68.16±0.37%和UV-A条件下的80.21±0.3%)。在可见和UV-A照明之间观察到毒性没有显着差异。此外,通过测量氧化应激和抗氧化酶活性来证实毒性数据。当在两种照明下与Tc混合时,独立的动作模型显示出较低浓度的N-TiO_2和较高浓度N-TiO_2的抗拮抗作用。对于4mgL〜(-1)N-TiO_2和1mg L〜(-1)TC的较高混合浓度,TC除去的百分比为约55.29%,并发现相应的TOC去除率为54.29%在可见和UV-A照明下31.04%。小球藻SP的ROS生成的网站。用电子转移链抑制剂鉴定。线粒体和叶绿体均以Collarla SP中的ROS产生的迹象。在暴露于N-TiO_2和混合物时,藻类细胞的SEM图像显示细胞的聚集和细胞膜的变形。

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