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首页> 外文期刊>Environmental research >Extended-spectrum beta-lactamase-producing Escherichia coli and antimicrobial resistance in municipal and hospital wastewaters in Czech Republic: Culture-based and metagenomic approaches
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Extended-spectrum beta-lactamase-producing Escherichia coli and antimicrobial resistance in municipal and hospital wastewaters in Czech Republic: Culture-based and metagenomic approaches

机译：在捷克共和国市和医院废水中产生大肠杆菌和抗微生物抗菌药物的延长谱β-内酰胺酶和抗微生物抗性

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摘要

Wastewaters serve as important hot spots for antimicrobial resistance and monitoring can be used to analyse the abundance and diversity of antimicrobial resistance genes at the level of large bacterial and human populations. In this study, whole genome sequencing of beta-lactamase-producing Escherichia coli and metagenomic analysis of whole-community DNA were used to characterize the occurrence of antimicrobial resistance in hospital, municipal and river waters in the city of Brno (Czech Republic). Cefotaxime-resistant E. coli were mainly extended-spectrum beta-lactamase (ESBL) producers (95.6%, n = 158), of which the majority carried bla_(CTX-M) (98.7%; n = 151) and were detected in all water samples except the outflow from hospital wastewater treatment plant. A wide phylogenetic diversity was observed among the sequenced E. coli (n = 78) based on the detection of 40 sequence types and single nucleotide polymorphisms (average number 34,666 ± 15,710) between strains. The metagenomic analysis revealed a high occurrence of bacterial genera with potentially pathogenic members, including Pseudomonas, Escherichia, Klebsiella, Aeromonas, Enterobacter and Arcobacter (relative abundance >50%) in untreated hospital and municipal wastewaters and predominance of environmental bacteria in treated and river waters. Genes encoding resistance to amino-glycosides, beta-lactams, quinolones and macrolides were frequently detected, however bla_(CTX-M) was not found in this dataset which may be affected by insufficient sequencing depth of the samples. The study pointed out municipal treated wastewater as a possible source of multi-drug resistant E. coli and antimicrobial resistance genes for surface waters. Moreover, the combination of two different approaches provided a more holistic view on antimicrobial resistance in water environments. The culture-based approach facilitated insight into the dynamics of ESBL-producing E. coli and the metagenomics shows abundance and diversity of bacteria and antimicrobial resistance genes vary across water sites.
机译：废水器作为抗微生物抗性和监测的重要热点，可用于分析大细菌和人口水平的抗微生物抗性基因的丰度和多样性。在该研究中，使用全群β-内酰胺酶的大肠杆菌和甲虫分析的全部基因组测序来表征在布尔诺市（捷克共和国）的医院，市政和河流中的抗微生物抗性发生。 CEFotaxime抗性大肠杆菌主要是扩展β-内酰胺酶（ESBL）生产商（95.6％，n = 158），其中大多数载有BLA_（CTX-M）（98.7％; n = 151）并被检测到除了医院废水处理厂外外部的所有水样。基于测定的大肠杆菌（n = 78），在菌株之间的检测和单核苷酸多态性（平均数34,666±15,710）中，在测序的大肠杆菌（n = 78）中观察到宽的系统发育多样性。聚丙烯瘤分析揭示了具有潜在病原构件的细菌属，包括假鼠，大肠杆菌，Klebsiella，Aeromonas，肠杆菌和Arcobacter（相对丰度> 50％），在未经处理的医院和市政废水中以及治疗和河水中的环境细菌的优势。经常检测到对氨基 - 糖苷，β-内酰胺，喹诺酮和大溴化胶剂的抗性的基因经常检测到，但在该数据集中未发现Bla_（CTX-M），其可能受到样品的序列不足的影响。该研究指出，城市治疗废水作为表面水域的多药物抗性大肠杆菌和抗微生物抗性基因来源。此外，两种不同方法的组合为水环境中的抗微生物抗性提供了更全面的观点。基于文化的方法促进了对ESBL制剂大肠杆菌的动态的洞察力，并且偏心神经显示出细菌和抗微生物抗性基因的丰富和多样性在水位上变化。

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  • 来源
    《Environmental research》 |2021年第2期|110487.1-110487.11|共11页
  • 作者

    Iva Kutilova; Matej Medvecky; Pimlapas Leekitcharoenphon; Patrick Munk; Martina Masarikova; Lenka Davidova-Gerzova; Ivana Jamborova; Valeria Bortolaia; Sunje J. Pamp; Monika Dolejska;

  • 作者单位

    CEITEC VFU University of Veterinary and Pharmaceutical Sciences Brno Palackeho tr. 1946/1 612 42 Brno Czech Republic Department of Biology and Wildlife Diseases Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Brno Palackeho tr. 1946/1 612 42 Brno Czech Republic;

    CEITEC VFU University of Veterinary and Pharmaceutical Sciences Brno Palackeho tr. 1946/1 612 42 Brno Czech Republic Biomedical Center Faculty of Medicine Charles University alej Svobody 1655/76 323 00 Plzen Czech Republic;

    Research Group for Genomic Epidemiology National Food Institute Technical University of Denmark Kgs. Lyngby 2800 Denmark;

    Research Group for Genomic Epidemiology National Food Institute Technical University of Denmark Kgs. Lyngby 2800 Denmark;

    CEITEC VFU University of Veterinary and Pharmaceutical Sciences Brno Palackeho tr. 1946/1 612 42 Brno Czech Republic Department of Infectious Diseases and Microbiology Faculty of Veterinary Medicine University of Veterinary and Pharmaceutical Sciences Brno Palackeho tr. 1946/1 612 42 Brno Czech Republic;

    CEITEC VFU University of Veterinary and Pharmaceutical Sciences Brno Palackeho tr. 1946/1 612 42 Brno Czech Republic Department of Infectious Diseases and Microbiology Faculty of Veterinary Medicine University of Veterinary and Pharmaceutical Sciences Brno Palackeho tr. 1946/1 612 42 Brno Czech Republic;

    CEITEC VFU University of Veterinary and Pharmaceutical Sciences Brno Palackeho tr. 1946/1 612 42 Brno Czech Republic;

    Research Group for Genomic Epidemiology National Food Institute Technical University of Denmark Kgs. Lyngby 2800 Denmark;

    Research Group for Genomic Epidemiology National Food Institute Technical University of Denmark Kgs. Lyngby 2800 Denmark;

    CEITEC VFU University of Veterinary and Pharmaceutical Sciences Brno Palackeho tr. 1946/1 612 42 Brno Czech Republic Department of Biology and Wildlife Diseases Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Brno Palackeho tr. 1946/1 612 42 Brno Czech Republic Biomedical Center Faculty of Medicine Charles University alej Svobody 1655/76 323 00 Plzen Czech Republic;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    B. coli; Wastewater treatment plants; ESBt; Whole genome sequencing;

    机译：B. Coli;污水处理厂;esbt;全基因组测序;

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