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首页> 外文期刊>Environmental research >Molecular characterization of Cryptococcus neoformans and Cryptococcus gattii from environmental sources and genetic comparison with clinical isolates in Apulia, Italy
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Molecular characterization of Cryptococcus neoformans and Cryptococcus gattii from environmental sources and genetic comparison with clinical isolates in Apulia, Italy

机译：来自意大利环境的新型隐球菌和加蒂隐球菌的分子表征以及与临床分离株的遗传比较

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摘要

The present study investigated the environmental distribution of Cryptococcus neoformans and C. gattii species complex molecular types, mating types and sequence types in Apulia, a region of Southern Italy. A total of 2078 specimens from arboreal and animal sources were analyzed. The percentage of positive samples was similar among both arboreal and animal specimens: 4.2% vs. 5.1% for C. neoformans species complex and 0.6% vs. 1.4% for C. gattii species complex. Molecular typing identified 78 isolates as VNI (76 ctA and two aA), one as AD-hybrid αADa, and 16 as VGI aB. VNI isolates presented 10 different sequence types (STs) and VGI isolates two. The most frequent STs among C. neoformans and C. gattii species complex isolates were ST23 (51%) and ST156 (90%), respectively. Comparison with molecular types and STs results obtained from 21 clinical isolates collected in Apulia showed that one C. neoformans VNI clinical isolate shared an identical sequence type of one arboreal isolate (ST61) and that one C. gattii VGI clinical isolate matched with the main ST (ST156) present in the environment. In addition, molecular type VNIV was found only among clinical isolates and was absent in the investigated environmental area. In conclusion, the present study identified which C. neoformans and C. gattii species complex genotypes are circulating in Apulia, defined their ecological niches and revealed the relationship with clinical cases. It represents a basal study for addressing future investigations and public health interventions in the region.
机译：本研究调查了意大利南部阿普利亚地区的新型隐球菌和加蒂梭菌物种复杂分子类型，交配类型和序列类型的环境分布。总共分析了2078个来自树木和动物来源的标本。树木和动物标本中阳性样本的百分比相似：新甲壳虫物种复合体的比例为4.2％，而新甲壳虫物种复合体的比例为5.1％，而加迪角藻物种复合体的比例为0.6％。分子分型鉴定出78个分离株为VNI（76 ctA和2个aa），一个为AD杂交αADa，16个为VGI aB。 VNI分离株呈现10种不同的序列类型（ST），VGI分离株呈现2种。在新隐梭菌和加蒂梭菌物种复合物分离株中最常见的STs分别是ST23（51％）和ST156（90％）。与从普利亚收集的21种临床分离株获得的分子类型和ST的比较结果表明，一种新孢子虫VNI临床分离株具有相同的序列类型，其中一种树突分离株（ST61）具有相同的序列类型，并且一种C. gattii VGI临床分离株与主要ST匹配。 （ST156）存在于环境中。此外，仅在临床分离株中发现了分子型VNIV，在研究的环境领域中不存在分子型。综上所述，本研究确定了普利亚正在传播哪些新孢子虫和加蒂弧菌物种复杂的基因型，确定了它们的生态位，并揭示了与临床病例的关系。它是针对该地区未来的调查和公共卫生干预措施的基础研究。

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  • 来源
    《Environmental research》 |2018年第1期|347-352|共6页
  • 作者

    Maria Teresa Montagna; Antonella De Donno; Giuseppina Caggiano; Francesca Serio; Osvalda De Giglio; Francesco Bagordo; R. DAmicis; Shawn R. Lockhart; Massimo Cogliati;

  • 作者单位

    Department of Biomedical Science and Human Oncology, University of Bari 'Aldo Mow', Bari, Italy;

    Department of Biological and Environmental Science and Technology, University of Salento, Lecce, Italy;

    Department of Biomedical Science and Human Oncology, University of Bari 'Aldo Mow', Bari, Italy;

    Department of Biological and Environmental Science and Technology, University of Salento, Lecce, Italy;

    Department of Biomedical Science and Human Oncology, University of Bari 'Aldo Mow', Bari, Italy;

    Department of Biological and Environmental Science and Technology, University of Salento, Lecce, Italy;

    Department of Biomedical Sciences for Health, Universita degli Studi di Muano, Milano, Italy;

    Fungal Reference Laboratory, Centers for Disease Control and Prevention, Atlanta, GA, USA;

    Department of Biomedical Sciences for Health, Universita degli Studi di Muano, Milano, Italy;

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