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Phytobeds with Fimbristylis dichotoma and Ammannia baccifera for treatment of real textile effluent: An in situ treatment, anatomical studies and toxicity evaluation

机译:含双歧杆菌和百日草的植物床处理真实的纺织品废水:原位处理,解剖学研究和毒性评估

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摘要

Fimbristylis dichotoma, Ammannia baccifera and their co-plantation consortium FA independently degraded Methyl Orange, simulated dye mixture and real textile effluent. Wild plants of F. dichotoma and A. baccifera with equal biomass showed 91% and 89% decolorization of Methyl Orange within 60 h at a concentration of 50 ppm, while 95% dye removal was achieved by consortium FA within 48 h. Floating phyto-beds with co-plantation (F. dichotoma and A. baccifera) for the treatment of real textile effluent in a constructed wetland was observed to be more efficient and achieved 79%, 72%, 77%, 66% and 56% reductions in ADMI color value, COD, BOD, TDS and TSS of textile effluent, respectively. HPTLC, GC-MS, FTIR, UV-vis spectroscopy and activated oxido-reductive enzyme activities confirmed the phytotrasformation of parent dye in to new metabolites. T-RFLP analysis of rhizospheric bacteria of F. dichotoma, A. baccifera and consortium FA revealed the presence of 88, 98 and 223 genera which could have been involved in dye removal. Toxicity evaluation of products formed after phyto-transformation of Methyl Orange by consortium FA on bivalves LameUidens marginaks revealed less damage of the gills architecture when analyzed histologically. Toxicity measurement by Random Amplification of Polymorphic DNA (RAPD) technique revealed bivalve DNA banding pattern in treated Methyl Orange sample suggesting less toxic nature of phytotransformed dye products.
机译:Fimbristylis dichotoma,Ammannia baccifera及其共同种植的联合会FA分别降解了甲基橙,模拟染料混合物和真实的纺织品废水。具有相等生物量的双歧F. dichotoma和B. abacifera野生植物在60 h内浓度为50 ppm时显示91%和89%的甲基橙脱色,而财团FA在48 h内实现了95%的脱色。观察到,在人工湿地中,通过人工种植的浮游植物床(F. dichotoma和A. baccifera)处理真正的纺织品废水更为有效,达到了79%,72%,77%,66%和56%分别降低纺织品废水的ADMI颜色值,COD,BOD,TDS和TSS。 HPTLC,GC-MS,FTIR,紫外可见光谱和活化的氧化还原酶活性证实了母体染料的植物转化为新的代谢产物。 T.RFLP分析双歧镰刀菌,双歧杆菌和FA的根际细菌表明存在88、98和223属,这可能与染料去除有关。组织学分析发现,财团FA对甲基橙进行植物转化后形成的产物对双壳类LameUidens marginaks的毒性评估显示,architecture结构受到的损害较小。通过多态性DNA随机扩增(RAPD)技术进行的毒性测量显示,处理过的甲基橙样品中双壳类DNA带状分布,表明植物转化染料产品的毒性较小。

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