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首页> 外文期刊>Environmental research >miRNA expression profiles and retinal blood vessel calibers are associated with short-term particulate matter air pollution exposure
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miRNA expression profiles and retinal blood vessel calibers are associated with short-term particulate matter air pollution exposure

机译：miRNA表达谱和视网膜血管口径与短期颗粒物空气污染暴露相关

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Introduction: Air pollution. a risk factor for cardiovascular diseases, can exert its effects through the microcirculation. Retinal blood vessel width is considered a marker for microvascular health and is associated with short-term PM_(10) exposure. microRNAs are key regulators of complex biological processes in cardiovascular health and disease and miRNA expression can be affected by air pollution exposure. Studies investigating the effect of ambient air pollution exposure on miRNA expression in combination with an assessment of the microvasculature do not exist. Methods: 50 healthy adults (50% women, 23-58 years old) were examined once a month from December 2014 until April 2015 in Flanders (Belgium). Fundus photos and venous blood samples were collected during the study visits. PM_(10) data were obtained from a nearby monitoring station. Image analysis was used to calculate the width of retinal blood vessels, represented as the Central Retinal Arteriolar/Venular Equivalent (CRAE/CRVE). Total miRNA was isolated from blood and the expression of miR-21, -146a and, -222 were measured using quantitative real-time PCR. Mixed models were used for statistical analysis. Results: Each short-term increase of 10 μg/m~3 PM_(10) during the 24 h preceding the study visit was associated with a 0.58 μm decrease (95% CI: -1.16, -0.0005; p = 0.056) in CRAE, a 0.99 μm increase (95% CI: 0.18, 1.80: p = 0.021) in CRVE, a 6.6% decrease (95% CI: -11.07, -2.17; p = 0.0038) in miR-21 expression and a 6.7% decrease (95% CI: -10.70, -2.75; p=0.0012) in miR-222 expression. Each 10% increase in miR-21 was associated with a 0.14 μm increase (95% CI: 0.0060, 0.24; p = 0.046) in CRAE whereas a similar increase in miR-222 expression was associated with a 0.28 μm decrease (95% CI: -0.50, -0.062; p = 0.016) in CRVE. These associations were also found in exposure windows ranging from 2 h to 1 week. Finally, we observed that the association between PM_(10) exposure and CRAE was mediated by miRNA-21 expression. Conclusion: PM_(10) exposure was associated with retinal arteriolar narrowing and venular widening. PM_(10) exposure affected miRNAs that are involved in inflammatory and oxidative stress pathways. We suggest that miRNA changes may be relevant to explain the association between PM_(10) and retinal vessel calibers.
机译：简介：空气污染。心血管疾病的危险因素，可以通过微循环发挥作用。视网膜血管宽度被认为是微血管健康的标志，并且与短期PM_（10）暴露有关。 microRNA是心血管健康和疾病中复杂生物过程的关键调节器，而暴露于空气污染中可能会影响miRNA的表达。不存在研究环境空气污染暴露对miRNA表达的影响以及对微脉管系统评估的研究。方法：从2014年12月至2015年4月，在比利时的法兰德斯（Flanders），每月对50名健康成人（50％的女性，23-58岁）进行一次检查。在研究访问期间收集了眼底照片和静脉血样本。 PM_（10）数据是从附近的监测站获得的。图像分析用于计算视网膜血管的宽度，表示为视网膜中央小动脉/静脉当量（CRAE / CRVE）。从血液中分离总miRNA，并使用实时定量PCR检测miR-21，-146a和-222的表达。混合模型用于统计分析。结果：在研究访问之前的24小时内，每短期增加10μg/ m〜3 PM_（10），CRAE就会降低0.58μm（95％CI：-1.16，-0.0005； p = 0.056）。 ，CRVE增加0.99μm（95％CI：0.18，1.80：p = 0.021），miR-21表达减少6.6％（95％CI：-11.07，-2.17; p = 0.0038），减少6.7％ （95％CI：-10.70，-2.75; p = 0.0012）在miR-222表达中。 miR-21每增加10％，CRAE就会增加0.14μm（95％CI：0.0060，0.24; p = 0.046），而miR-222表达的相似增加会减少0.28μm（95％CI） ：-0.50，-0.062; p = 0.016）。在从2小时到1周的暴露窗口中也发现了这些关联。最后，我们观察到PM_（10）暴露与CRAE之间的关联是由miRNA-21表达介导的。结论：PM_（10）暴露与视网膜小动脉狭窄和静脉扩大有关。 PM_（10）暴露会影响涉及炎症和氧化应激途径的miRNA。我们建议，miRNA的变化可能与解释PM_（10）和视网膜血管口径之间的关联有关。

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  • 来源
    《Environmental research》 |2016年第5期|24-31|共8页
  • 作者

    Tijs Louwies; Caroline Vuegen; Luc Int Panis; Bianca Cox; Karen Vrijens; Tim S. Nawrot; Patrick De Boever;

  • 作者单位

    Environmental Risk and Health, Flemish Institute for Technological Research (VITO), Mol, Belgium,Centre for Environmental Sciences, Hasselt University, Hasselt, Belgium;

    Centre for Environmental Sciences, Hasselt University, Hasselt, Belgium;

    Environmental Risk and Health, Flemish Institute for Technological Research (VITO), Mol, Belgium,Transportation Research Institute, Hasselt University, Hasselt, Belgium;

    Centre for Environmental Sciences, Hasselt University, Hasselt, Belgium;

    Centre for Environmental Sciences, Hasselt University, Hasselt, Belgium;

    Centre for Environmental Sciences, Hasselt University, Hasselt, Belgium,Department of Public Health & Primary Care, Leuven University, Leuven, Belgium;

    Environmental Risk and Health, Flemish Institute for Technological Research (VITO), Mol, Belgium,Centre for Environmental Sciences, Hasselt University, Hasselt, Belgium,VITO, Vlasmeer 7, 2400 Mol, Belgium;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Air pollution; Retina; Microvascular changes; miRNA expression;

    机译：空气污染;视网膜;微血管变化;miRNA表达;

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