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Molecular detection of Giardia contamination in water bodies in a zoo

机译：动物园水体中贾第鞭毛虫污染的分子检测

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摘要

We used a combined microscopy-molecular approach to determine the occurrence and identities of waterborne Giardia sp. cysts isolated from 18 separate, 101 grab samples collected from a Malaysian zoo. Microscopy revealed that 17 of 18 samples were Giardia cyst positive with concentrations ranging from 1 to 120 cysts/l. Nine (52.9%) of the 17 cyst positive samples produced amplicons of which 7 (77.8%) could be sequenced. Giardia duodenalis assemblage A (6 of 7) and assemblage B (1 of 7), both infectious to humans, were identified at all sampling sites at the zoo. The presence of human infectious cysts raises public health issues, and their occurrence, abundance and sources should be investigated further. In this zoo setting, our data highlight the importance of incorporating environmental sampling (monitoring) in addition to routine faecal examinations to determine veterinary and public health risks, and water monitoring should be considered for inclusion as a separate element in hazard analysis, as it often has a historical (accumulative) connotation.
机译：我们使用组合的显微镜-分子方法来确定水性贾第鞭毛虫的发生和身份。从一个马来西亚动物园收集的18个独立的101个样本中分离出的囊肿。显微镜检查显示18个样本中有17个为贾第虫囊肿阳性，浓度范围为1至120个囊肿/ l。 17个囊肿阳性样品中有9个（52.9％）产生了扩增子，其中7个（77.8％）可以测序。在动物园的所有采样点都发现了对人有感染力的贾第鞭毛虫组合A（7个中的6个）和B组（7个中的1个）。人类感染性囊肿的存在引发了公共卫生问题，应进一步调查它们的发生，丰度和来源。在这种动物园环境中，我们的数据强调了除了常规粪便检查以外，还应包括环境采样（监测）以确定兽医和公共健康风险的重要性，并且应将水监测作为危险分析中的一个独立要素考虑在内，因为它经常具有历史（累积）内涵。

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  • 来源
    《Environmental research》 |2009年第7期|857-859|共3页
  • 作者

    Yvonne A.L. Lim; Monica M. Lai; Mohammed A.K. Mahdy; H.R. Mat Nairn; Huw V. Smith;

  • 作者单位

    Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;

    Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;

    Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia Department of Parasitology, Faculty of Medicine, Sana'a University, Sana'a, Yemen;

    Hospital & Veterinary Services, Zoo Negara Malaysia, Ulu Klang 68000 Ampang, Kuala Lumpur, Malaysia;

    Scottish Parasite Diagnostic Laboratory, Stobhill Hospital, Glasgow G21 3UW, Scotland, UK;

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  • 正文语种 eng
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  • 关键词

    giardia duodenalis cysts; assemblages A and B; environment; zoo; malaysia;

    机译：十二指肠贾第虫囊肿;组合A和B;环境;动物园;马来西亚;

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