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首页> 外文期刊>Environmental Science & Technology >Bacterial Survival Strategies in an Alkaline Tailing Site and the Physiological Mechanisms of Dominant Phylotypes As Revealed by Metagenomic Analyses
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Bacterial Survival Strategies in an Alkaline Tailing Site and the Physiological Mechanisms of Dominant Phylotypes As Revealed by Metagenomic Analyses

机译:元基因组分析揭示了碱性尾矿中细菌的生存策略和优势型的生理机制。

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摘要

Microorganisms inhabiting mine tailings require specific metabolic strategies to survive, which may hold potential for pollution clean up. Effective in situ bioremediation will rely on an in-depth understanding of the function of the bacterial communities, especially the abundant and metabolically active phylotypes. In this study, the bacterial communities collected from an alkaline tailing site were profiled by 16S rRNA gene amplicon sequencing as well as shotgun metagenomic analysis. Our results indicated that potentials for carbon and nitrogen fixation as well as metal resistance and transformation were widespread among the bacterial community members, especially in highly enriched phylotypes, such as members of Thiobacillus and Meiothermus. Important functional microbial guilds including carbon and nitrogen fixers may contribute to phytoremediation by providing nutrients for hyperaccumulator plants. In addition, metal-metabolizing bacteria may influence metal speciation and solubility. This discovery provides an understanding for microbial survival strategies in the tailings and lays the foundation for future potential manipulation of the tailing microbiome for in situ bioremediation.
机译:居住在矿山尾矿中的微生物需要特定的代谢策略才能生存,这可能具有清除污染的潜力。有效的原位生物修复将取决于对细菌群落功能的深入了解,尤其是丰富的和具有代谢活性的系统型。在这项研究中,通过16S rRNA基因扩增子测序以及shot弹枪宏基因组分析,对从碱性尾矿位点收集的细菌群落进行了分析。我们的结果表明,碳和氮固定以及金属抗性和转化的潜力在细菌群落成员中广泛存在,特别是在高度富集的系统型中,例如硫杆菌和嗜热菌的成员。重要的功能性微生物协会(包括碳和氮固定剂)可能会通过为过度蓄积植物提供营养来促进植物修复。另外,金属代谢细菌可能会影响金属形态和溶解度。该发现提供了对尾矿中微生物生存策略的理解,并为将来对尾矿微生物组进行原位生物修复的潜在操作奠定了基础。

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  • 来源
    《Environmental Science & Technology》 |2018年第22期|13370-13380|共11页
  • 作者单位

    Rutgers State Univ, Dept Environm Sci, New Brunswick, NJ 08901 USA;

    Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA;

    Guangzhou Univ, Key Lab Water Qual & Conservat Pearl River Delta, Sch Environm Sci & Engn, Minist Educ, Guangzhou 510006, Guangdong, Peoples R China;

    Rutgers State Univ, Dept Biochem & Microbiol, New Brunswick, NJ 08901 USA;

    Guangdong Inst Ecoenvironm Sci & Technol, Guangdong Key Lab Integrated Agroenvironm Pollut, Guangzhou 510650, Guangdong, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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