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首页> 外文期刊>Environmental Science & Technology >Unraveling Microbial Communities Associated with Methylmercury Production in Paddy Soils
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Unraveling Microbial Communities Associated with Methylmercury Production in Paddy Soils

机译：稻田土壤中与甲基汞生产相关的微生物群落的研究

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摘要

Rice consumption is now recognized as an important pathway of human exposure to the neurotoxin methylmercury (MeHg), particularly in countries where rice is a staple food. Although the discovery of a two-gene cluster hgcAB has linked Hg methylation to several phylogenetically diverse groups of anaerobic microorganisms converting inorganic mercury (Hg) to MeHg, the prevalence and diversity of Hg methylators in microbial communities of rice paddy soils remain unclear. We characterized the abundance and distribution of hgcAB genes using third-generation PacBio long-read sequencing and Illumina short-read metagenomic sequencing, in combination with quantitative PCR analyses in several mine-impacted paddy soils from southwest China. Both Illumina and PacBio sequencing analyses revealed that Hg methylating communities were dominated by iron-reducing bacteria (i.e., Geobacter) and methanogens, with a relatively low abundance of hgcA(+) sulfate-reducing bacteria in the soil. A positive correlation was observed between the MeHg content in soil and the relative abundance of Geobacter carrying the hgcA gene. Phylogenetic analysis also uncovered some hgcAB sequences closely related to three novel Hg methylators, Geobacter anodireducens, Desulfuromonas sp. DDH964, and Desulfovibrio sp. J2, among which G. anodireducens was validated for its ability to methylate Hg. These findings shed new light on microbial community composition and major clades likely driving Hg methylation in rice paddy soils.
机译：如今，大米消费被认为是人类接触神经毒素甲基汞（MeHg）的重要途径，特别是在大米为主要食品的国家。尽管两个基因簇hgcAB的发现已将Hg甲基化与几个将厌氧微生物从无机汞（Hg）转化为MeHg的系统发育多样性的厌氧微生物组联系起来，但Hg甲基化因子在稻田土壤微生物群落中的流行和多样性仍然不清楚。我们使用第三代PacBio长读测序和Illumina短读宏基因组测序，结合定量PCR分析，在中国西南部几种受地雷影响的水稻土中表征了hgcAB基因的丰度和分布。 Illumina和PacBio测序分析均显示，汞还原甲基化细菌（即Geobacter）和产甲烷菌占主导地位的Hg甲基化群落，土壤中hgcA（+）硫酸盐还原菌的含量相对较低。土壤中的甲基汞含量与携带hgcA基因的土壤杆菌的相对丰度之间存在正相关。系统发育分析还发现了一些与三个新型汞甲基化剂，厌氧性土杆菌，脱硫尿单胞菌密切相关的hgcAB序列。 DDH964和Desulfovibrio sp。 J2，其中G. anodireducens被验证具有甲基化Hg的能力。这些发现为水稻群落中的微生物群落组成和可能促进汞甲基化的主要进化枝提供了新的思路。

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  • 来源
    《Environmental Science & Technology》 |2018年第22期|13110-13118|共9页
  • 作者

    Liu Yu-Rong; Johs Alexander; Bi Li; Lu Xia; Hu Hang-Wei; Sun Dan; He Ji-Zheng; Gu Baohua;

  • 作者单位

    Univ Melbourne, Dept Vet & Agr Sci, Melbourne, Vic 3004, Australia;

    Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China;

    Zhejiang Univ, Ocean Coll, Hangzhou 310058, Zhejiang, Peoples R China;

    Oak Ridge Natl Lab, Environm Sci Div, Oak Ridge, TN 37831 USA;

    Univ Tennessee, Dept Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA;

    Huazhong Agr Univ, Coll Resources & Environm, Wuhan 430070, Hubei, Peoples R China;

    Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing Shi 100085, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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