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首页> 外文期刊>Environmental Science & Technology >Normalized Quantitative PCR Measurements as Predictors for Ethene Formation at Sites Impacted with Chlorinated Ethenes
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Normalized Quantitative PCR Measurements as Predictors for Ethene Formation at Sites Impacted with Chlorinated Ethenes

机译：标准化的定量PCR测量作为预测受氯化乙烯影响的部位乙烯形成的指标

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Quantitative PCR (qPCR) targeting Dehalococ-coides mccartyi (Dhc) biomarker genes supports effective management at sites impacted with chlorinated ethenes. To establish correlations between Dhc biomarker gene abundances and ethene formation (i.e., detoxification), 859 groundwater samples representing 62 sites undergoing monitored natural attenuation or enhanced remediation were analyzed. Dhc 16S rRNA genes and the vinyl chloride (VC) reductive dehalogenase genes bvcA and vcrA were detected in 88% and 61% of samples, respectively, from wells with ethene. Dhc 16S rRNA, bvcA, vcrA, and tceA (implicated in cometabolic reductive VC dechlorination) gene abundances all positively correlated with ethene formation. Significantly greater ethene concentrations were observed when Dhc 16S rRNA gene and VC RDase gene abundances exceeded 10(7) and 10(6) copies L-1, respectively, and when Dhc 16S rRNA- and bvcA + vcrA-to-total bacterial 16S rRNA gene ratios exceeded 0.1%. Dhc 16S rRNA gene-to-vcrA/bvcA ratios near unity also indicated elevated ethene; however, no increased ethene was observed in 19 wells where vcrA and/or bvcA gene copy numbers exceeded Dhc cell numbers 10- to 10 000-fold. Approximately one-third of samples with detectable ethene lacked bvcA, vcrA, and tceA, suggesting that comprehensive understanding of VC detoxification biomarkers has not been achieved. Although the current biomarker suite is incomplete, the data analysis corroborates the value of the available Dhc DNA biomarkers for prognostic and diagnostic groundwater monitoring at sites impacted with chlorinated ethenes.
机译：靶向Dehalococ-coides mccartyi（Dhc）生物标记基因的定量PCR（qPCR）支持在受氯化乙烯影响的位点进行有效管理。为了建立Dhc生物标志物基因丰度与乙烯形成（即解毒）之间的相关性，分析了代表62个站点的859个地下水样品，这些站点正在受到监测的自然衰减或增强修复作用。在带有乙烯的孔中，分别在88％和61％的样品中检测到Dhc 16S rRNA基因和氯乙烯（VC）还原性脱卤素酶基因bvcA和vcrA。 Dhc 16S rRNA，bvcA，vcrA和tceA（与代谢还原性VC脱氯有关）基因丰度均与乙烯形成呈正相关。当Dhc 16S rRNA基因和VC RDase基因的丰度分别超过L-1的10（7）和10（6）拷贝时，以及当Dhc 16S rRNA和bvcA + vcrA占细菌总数16S rRNA时，乙烯浓度显着增加。基因比例超过0.1％。 Dhc 16S rRNA基因与vcrA / bvcA的比率接近统一也表明乙烯含量升高；然而，在19口井中没有观察到乙烯的增加，其中vcrA和/或bvcA基因拷贝数超过了Dhc细胞数的10到10000倍。大约有可检测到的乙烯的样品中有三分之一缺乏bvcA，vcrA和tceA，这表明尚未完全了解VC排毒生物标志物。尽管当前的生物标志物套件还不完整，但数据分析证实了可用的Dhc DNA生物标志物在受氯化乙烯影响的地点进行预后和诊断地下水监测的价值。

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  • 来源
    《Environmental Science & Technology》 |2018年第22期|13410-13420|共11页
  • 作者

    Clark Katherine; Taggart Dora M.; Baldwin Brett R.; Ritalahti Kirsti M.; Murdoch Robert W.; Hatt Janet K.; Loffler Frank E.;

  • 作者单位

    Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN 37996 USA;

    Sch Civil & Environm Engn, Atlanta, GA 30332 USA;

    Microbial Insights Inc, 10515 Res Dr, Knoxville, TN 37932 USA;

    Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37996 USA;

    Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA;

    Univ Tennessee, Dept Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA;

    Oak Ridge Natl Lab, JIBS, Oak Ridge, TN 37831 USA;

    Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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