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Adsorption of Methylmercury onto Geobacter bemidijensis Bem

机译：甲基汞吸附于贝米吉斯杆菌Gembacter Bemijensis Bem上

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摘要

The anaerobic bacterium Geobacter bemidijensis Bern has the unique ability to both produce and degrade methylmercury (MeHg). While the adsorption of MeHg onto bacterial surfaces can affect the release of MeHg into aquatic environments as well as the uptake of MeHg for demethylation, the binding of MeHg to the bacterial envelope remains poorly understood. In this study, we quantified the adsorption of MeHg onto G. bemidijensis and applied X-ray absorption spectroscopy (XAS) to elucidate the mechanism of MeHg binding. The results showed MeHg adsorption onto G. bemidijensis cell surfaces was rapid and occurred via complexation to sulfhydryl functional groups. Titration experiments yielded cell surface sulfhydryl concentrations of 3.8 +/- 0.2 mu mol/g (wet cells). A one-site adsorption model with MeHg binding onto sulfhydryl sites provided excellent fits to adsorption isotherms conducted at different cell densities. The log K binding constant of MeHg onto the sulfhydryl sites was determined to be 10.5 +/- 0.4. These findings provide a quantitative framework to describe MeHg binding onto bacterial cell surfaces and elucidate the importance of bacterial cells as possible carriers of adsorbed MeHg in natural aquatic systems.
机译：厌氧细菌伯米氏菌伯尔尼具有产生和降解甲基汞（MeHg）的独特能力。尽管MeHg在细菌表面的吸附会影响MeHg在水生环境中的释放以及甲基化对MeHg的吸收，但对MeHg与细菌包膜的结合仍然知之甚少。在这项研究中，我们量化了MeHg在bemidijensis上的吸附，并应用X射线吸收光谱（XAS）阐明了MeHg结合的机理。结果表明，MeHg吸附在Bemidijensis细胞表面的速度很快，并通过与巯基官能团的络合而发生。滴定实验产生的细胞表面巯基浓度为3.8 +/- 0.2μmol / g（湿细胞）。 MeHg结合到巯基位点的单点吸附模型提供了与在不同细胞密度下进行的吸附等温线的极佳拟合。测定MeHg在巯基位点上的log K结合常数为10.5 +/- 0.4。这些发现为描述甲基汞与细菌细胞表面的结合提供了定量框架，并阐明了细菌细胞作为天然水生系统中吸附的甲基汞的可能载体的重要性。

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  • 来源
    《Environmental Science & Technology》 |2018年第20期|11564-11572|共9页
  • 作者

    Wang Yuwei; Yu Qiang; Mishra Bhoopesh; Schaefer Jeffra K.; Fein Jeremy B.; Yee Nathan;

  • 作者单位

    Rutgers State Univ, Dept Environm Sci, New Brunswick, NJ 08901 USA;

    Univ Notre Dame, Dept Civil & Environm Engn & Earth Sci, Notre Dame, IN 46556 USA;

    Univ Leeds, Sch Chem & Proc Engn, Leeds LS2 9JT, W Yorkshire, England;

    Rutgers State Univ, Dept Environm Sci, New Brunswick, NJ 08901 USA;

    Univ Notre Dame, Dept Civil & Environm Engn & Earth Sci, Notre Dame, IN 46556 USA;

    Rutgers State Univ, Dept Environm Sci, New Brunswick, NJ 08901 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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