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首页> 外文期刊>Environmental Science & Technology >Environmental Fate of RNA Interference Pesticides: Adsorption and Degradation of Double-Stranded RNA Molecules in Agricultural Soils
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Environmental Fate of RNA Interference Pesticides: Adsorption and Degradation of Double-Stranded RNA Molecules in Agricultural Soils

机译：RNA干扰农药的环境命运：农业土壤中双链RNA分子的吸附和降解

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摘要

Double-stranded RNA (dsRNA) pesticides are a new generation of crop protectants that interfere with protein expression in targeted pest insects by a cellular mechanism called RNA interference (RNAi). The ecological risk assessment of these emerging pesticides necessitates an understanding of the fate of dsRNA molecules in receiving environments, among which agricultural soils are most important. We herein present an experimental approach using phosphorus-32 (P-32)-radiolabeled dsRNA that allows studying key fate processes of dsRNA in soils with unprecedented sensitivity. This approach resolves previous analytical challenges in quantifying unlabeled dsRNA and its degradation products in soils. We demonstrate that P-32-dsRNA and its degradation products are quantifiable at concentrations as low as a few nanograms of dsRNA per gram of soil by both Cerenkov counting (to quantify total P-32-activity) and by polyacrylamide gel electrophoresis followed by phosphorimaging (to detect intact P-32-dsRNA and its P-32-containing degradation products). We show that dsRNA molecules added to soil suspensions undergo adsorption to soil particle surfaces, degradation in solution, and potential uptake by soil microorganisms. The results of this work on dsRNA adsorption and degradation advance a process-based understanding of the fate of dsRNA in soils and will inform ecological risk assessments of emerging dsRNA pesticides.
机译：双链RNA（dsRNA）农药是新一代作物保护剂，可通过称为RNA干扰（RNAi）的细胞机制干扰目标害虫中的蛋白质表达。这些新兴农药的生态风险评估需要了解接收环境中dsRNA分子的命运，其中农业土壤最为重要。我们在这里提出了一种使用磷32（P-32）-放射性标记的dsRNA的实验方法，该方法允许以前所未有的敏感性研究dsRNA在土壤中的关键命运过程。这种方法解决了以前定量分析土壤中未标记的dsRNA及其降解产物的分析难题。我们证明，通过切伦科夫计数（定量总P-32活性）和聚丙烯酰胺凝胶电泳，然后进行磷光成像，P-32-dsRNA及其降解产物的浓度可低至每克土壤几纳克dsRNA。 （以检测完整的P-32-dsRNA及其含P-32的降解产物）。我们表明，添加到土壤悬浮液中的dsRNA分子会吸附到土壤颗粒表面，在溶液中降解，并可能被土壤微生物吸收。有关dsRNA吸附和降解的这项工作的结果促进了对土壤中dsRNA命运的基于过程的了解，并将为新兴dsRNA农药的生态风险评估提供依据。

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  • 来源
    《Environmental Science & Technology》 |2019年第6期|3027-3036|共10页
  • 作者

    Parker Kimberly M.; Borrero Veronica Barragan; van Leeuwen Daniel M.; Lever Mark A.; Mateescu Bogdan; Sander Michael;

  • 作者单位

    Washington Univ, Dept Energy Environm & Chem Engn, St Louis, MO 63130 USA|Swiss Fed Inst Technol, Inst Biogeochem & Pollutant Dynam, CH-8092 Zurich, Switzerland;

    Swiss Fed Inst Technol, Dept Biol, Inst Mol Plant Biol, CH-8092 Zurich, Switzerland;

    Swiss Fed Inst Technol, Dept Biol, Inst Mol Plant Biol, CH-8092 Zurich, Switzerland;

    Swiss Fed Inst Technol, Inst Biogeochem & Pollutant Dynam, CH-8092 Zurich, Switzerland;

    Swiss Fed Inst Technol, Dept Biol, Inst Mol Plant Biol, CH-8092 Zurich, Switzerland;

    Swiss Fed Inst Technol, Inst Biogeochem & Pollutant Dynam, CH-8092 Zurich, Switzerland;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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