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首页> 外文期刊>Environmental Science & Technology >Motility of Shewanella oneidensis MR-1 Allows for Nitrate Reduction in the Toxic Region of a Ciprofloxacin Concentration Gradient in a Microfluidic Reactor
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Motility of Shewanella oneidensis MR-1 Allows for Nitrate Reduction in the Toxic Region of a Ciprofloxacin Concentration Gradient in a Microfluidic Reactor

机译：沙瓦氏菌（Shewanella oneidensis）MR-1的运动性可在微流控反应器中降低环丙沙星浓度梯度的有毒区域中的硝酸盐含量。

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摘要

Subsurface environments often contain mixtures of contaminants in which the microbial degradation of one pollutant may be inhibited by the toxicity of another. Agricultural settings exemplify these complex environments, where antimicrobial leachates may inhibit nitrate bioreduction, and are the motivation to address this fundamental ecological response. In this study, a microfluidic reactor was fabricated to create diffusion controlled concentration gradients of nitrate and ciprofloxacin under anoxic conditions in order to evaluate the ability of Shewanella oneidenisis MR-1 to reduce the former in the presence of the latter. Results show a surprising ecological response, where swimming motility allow S. oneidensis MR-1 to accumulate and maintain metabolic activity for nitrate reduction in regions with toxic ciprofloxacin concentrations (i.e., SOX minimum inhibitory concentration, MIC), despite the lack of observed antibiotic resistance. Controls with limited nutrient flux and a nonmotile mutant (Of lag) show that cells cannot colonize antibiotic rich microenvironments, and this results in minimal metabolic activity for nitrate reduction. These results demonstrate that under anoxic, nitrate-reducing conditions, motility can control microbial habitability and metabolic activity in spatially heterogeneous toxic environments.
机译：地下环境通常包含污染物的混合物，其中一种污染物的微生物降解可能被另一种污染物的毒性所抑制。农业环境就是这些复杂环境的典范，在这些环境中，抗菌浸出液可能会抑制硝酸盐的生物还原，并且是解决这种基本生态反应的动力。在这项研究中，制造了微流体反应器，以在缺氧条件下产生硝酸盐和环丙沙星的扩散控制浓度梯度，以评估单核希瓦氏菌MR-1在存在后者的情况下还原前者的能力。结果显示出令人惊讶的生态响应，尽管缺乏观察到的抗生素抗性，但游泳运动使S. oneidensis MR-1积累并维持具有毒性环丙沙星浓度（即SOX最低抑菌浓度，MIC）的区域中硝酸盐还原的代谢活性。 。具有有限的养分通量和不活动的突变体（滞后）的对照显示，细胞无法在富含抗生素的微环境中定殖，这导致硝酸盐还原的代谢活性最小。这些结果表明，在缺氧，减少硝酸盐的条件下，运动性可以控制空间异质毒性环境中的微生物可居住性和代谢活性。

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  • 来源
    《Environmental Science & Technology》 |2019年第5期|2778-2787|共10页
  • 作者

    Alcalde Reinaldo E.; Michelson Kyle; Zhou Lang; Schmitz Emily V.; Deng Jinzi; Sanford Robert A.; Fouke Bruce W.; Werth Charles J.;

  • 作者单位

    Univ Texas Austin, Dept Civil Architectural & Environm Engn, 301 E Dean Keeton St, Austin, TX 78712 USA;

    Univ Texas Austin, Dept Civil Architectural & Environm Engn, 301 E Dean Keeton St, Austin, TX 78712 USA;

    Univ Texas Austin, Dept Civil Architectural & Environm Engn, 301 E Dean Keeton St, Austin, TX 78712 USA;

    Univ Texas Austin, McKetta Dept Chem Engn, 200 E Dean Keeton St, Austin, TX 78712 USA;

    Univ Illinois, Carl R Woese Inst Genom Biol, 1206 W Gregory Dr, Urbana, IL 61801 USA;

    Univ Illinois, Dept Geol, 1301 West Green St, Urbana, IL 61801 USA;

    Univ Illinois, Carl R Woese Inst Genom Biol, 1206 W Gregory Dr, Urbana, IL 61801 USA|Univ Illinois, Dept Geol, 1301 West Green St, Urbana, IL 61801 USA|Univ Illinois, Dept Microbiol, 601 South Goodwin Ave, Urbana, IL 61801 USA;

    Univ Texas Austin, Dept Civil Architectural & Environm Engn, 301 E Dean Keeton St, Austin, TX 78712 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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