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首页> 外文期刊>Environmental Science & Technology >Effect of Biochar on Microbial Growth: A Metabolomics and Bacteriological Investigation in E. coli
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Effect of Biochar on Microbial Growth: A Metabolomics and Bacteriological Investigation in E. coli

机译：生物炭对微生物生长的影响：大肠杆菌中的代谢组学和细菌学研究

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Biochar has been proposed as a soil amendment in agricultural applications due to its advantageous adsorptive properties, high porosity, and low cost. These properties allow biochar to retain soil nutrients, yet the effects of biochar on bacterial growth remain poorly understood. To examine how biochar influences microbial metabolism, Escherichia coli was grown in a complex, well-defined media and treated with either biochar or activated carbon. The concentration of metabolites in the media were then quantified at several time points using NMR spectroscopy. Several metabolites were immediately adsorbed by the char, including L-asparagine, L-glutamine, and L-arginine. However, we find that biochar quantitatively adsorbs less of these metabolic precursors when compared to activated carbon. Electron microscopy reveals differences in surface morphology after cell culture, suggesting that Escherichia coli can form biofilms on the surfaces of the biochar. An examination of significant compounds in the tricarboxylic acid cycle and glycolysis reveals that treatment with biochar is less disruptive than activated carbon throughout metabolism. While both biochar and activated carbon slowed growth compared to untreated media, Escherichia coli in biochar-treated media grew more efficiently, as indicated by a longer logarithmic growth phase and a higher final cell density. This work suggests that biochar can serve as a beneficial soil amendment while minimizing the impact on bacterial viability. In addition, the experiments identify a mechanism for biochar's effectiveness in soil conditioning and reveal how biochar can alter specific bacterial metabolic pathways.
机译：由于生物炭具有良好的吸附性能，高孔隙率和低成本，其已被提出作为农业应用中的土壤改良剂。这些特性使生物炭能够保留土壤养分，但是生物炭对细菌生长的影响仍然知之甚少。为了检查生物炭如何影响微生物的代谢，大肠杆菌在复杂的，定义明确的培养基中生长，并用生物炭或活性炭处理。然后使用NMR光谱在几个时间点对培养基中代谢物的浓度进行定量。焦炭立即吸收了几种代谢物，包括L-天冬酰胺，L-谷氨酰胺和L-精氨酸。但是，我们发现与活性炭相比，生物炭在数量上较少地吸收这些代谢前体。电子显微镜揭示了细胞培养后表面形态的差异，这表明大肠杆菌可以在生物炭的表面上形成生物膜。对三羧酸循环和糖酵解中重要化合物的检查显示，在整个代谢过程中，用生物炭处理的破坏性均不如活性炭。尽管与未经处理的培养基相比，生物炭和活性炭的生长都减慢了，但经生物炭处理的培养基中的大肠杆菌生长效率更高，这表现为更长的对数生长期和更高的最终细胞密度。这项工作表明，生物炭可以作为有益的土壤改良剂，同时将对细菌生存力的影响降至最低。此外，实验确定了生物炭在土壤调节中的作用机理，并揭示了生物炭如何改变特定的细菌代谢途径。

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  • 来源
    《Environmental Science & Technology》 |2019年第5期|2635-2646|共12页
  • 作者

    Hill Rebecca A.; Hunt John; Sanders Emily; Tran Melanie; Burk Griffin A.; Mlsna Todd E.; Fitzkee Nicholas C.;

  • 作者单位

    Mississippi State Univ, Dept Chem, Mississippi State, MS 39762 USA;

    Mississippi State Univ, Dept Chem, Mississippi State, MS 39762 USA;

    Mississippi State Univ, Dept Chem, Mississippi State, MS 39762 USA;

    Mississippi State Univ, Dept Chem, Mississippi State, MS 39762 USA;

    Mississippi State Univ, Dept Chem, Mississippi State, MS 39762 USA;

    Mississippi State Univ, Dept Chem, Mississippi State, MS 39762 USA;

    Mississippi State Univ, Dept Chem, Mississippi State, MS 39762 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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