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首页> 外文期刊>Environmental Science & Technology >Degradation and Deactivation of Bacterial Antibiotic Resistance Genes during Exposure to Free Chlorine, Monochloramine, Chlorine Dioxide, Ozone, Ultraviolet Light, and Hydroxyl Radical
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Degradation and Deactivation of Bacterial Antibiotic Resistance Genes during Exposure to Free Chlorine, Monochloramine, Chlorine Dioxide, Ozone, Ultraviolet Light, and Hydroxyl Radical

机译：暴露于游离氯，一氯胺，二氧化氯，臭氧，紫外线和羟基自由基的过程中细菌抗生素抗性基因的降解和失活

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摘要

This work investigated degradation (measured by qPCR) and biological deactivation (measured by culture based natural transformation) of extra- and intracellular antibiotic resistance genes (eARGs and iARGs) by free available chlorine (FAC), NH2Cl, O-3, ClO2, and UV light (254 nm), and of eARGs by (OH)-O-center dot, using a chromosomal ARG (blt) of multidrug-resistant Bacillus subtilis 1A189. Rate constants for degradation of four 266-1017 bp amplicons adjacent to or encompassing the acfA mutation enabling blt overexpression increased in proportion to #AT+GC bps/amplicon, or in proportion to #5'-GG-3' or 5'-TT-3' doublets/amplicon, with respective values ranging from 0.59 to 2.3 (x10(11) M-1 s(-1)) for (OH)-O-center dot, 1.8-6.9 (x10(4) s(-1)) for O-3, 3.9-9.2 (x10(3) M-1 s(-1)) for FAC, 0.35-1.2(x10(1) M-1 s(-1)) for ClO2, and 2.0-8.8 (x10(-2) cm(2)/mJ) for UV at pH 7, and from 1.7-4.4 M(-1)s(-1) for NH2Cl at pH 8. For FAC, NH2Cl, O-3, ClO2, and UV, ARG deactivation paralleled degradation of amplicons approximating a similar to 800-1000 bp acfA-flanking sequence required for natural transformation in B. subtilis, whereas deactivation outpaced degradation for (OH)-O-center dot. At practical disinfectant exposures, eARGs and iARGs were = 90% degraded/deactivated by FAC, O-3, and UV, but recalcitrant to NH2Cl and ClO2. iARG degradation/deactivation always lagged cell inactivation. These findings provide a quantitative framework for evaluating ARG fate during disinfection/oxidation, and support using qPCR as a proxy for tracking ARG deactivation under carefully selected circumstances.
机译：这项工作研究了游离有效氯（FAC），NH2Cl，O-3，ClO2和H2O3对细胞外和细胞内抗生素抗性基因（eARGs和iARGs）的降解（通过qPCR测量）和生物失活（通过基于培养的自然转化测量）。使用耐多药枯草芽孢杆菌1A189的染色体ARG（blt），通过（OH）-O-中心点的紫外线（254 nm）和eARGs。与acfA突变相邻或包围的四个266-1017 bp扩增子降解的速率常数，使blt过度表达与＃AT + GC bps /扩增子成比例，或与＃5'-GG-3'或5'-TT成比例增加-3'doublets / amplicon，（OH）-O-中心点的相应值范围从0.59到2.3（x10（11）M-1 s（-1）），1.8-6.9（x10（4）s（- 1）对于O-3，对于FAC为3.9-9.2（x10（3）M-1 s（-1）），对于ClO2为0.35-1.2（x10（1）M-1 s（-1）），以及2.0对于pH值为7的UV为-8.8（x10（-2）cm（2）/ mJ），对于pH为8的NH2Cl为1.7-4.4 M（-1）s（-1）。对于FAC，NH2Cl，O-3 ，ClO2和UV，ARG失活与扩增子的降解平行，近似于枯草芽孢杆菌自然转化所需的800-1000 bp acfA侧翼序列，而失活超过（OH）-O-中心点的降解。在实际的消毒剂暴露条件下，FAC，O-3和紫外线使eARGs和iARGs≥90％降解/失活，但难分解为NH2Cl和ClO2。 iARG降解/失活总是滞后于细胞失活。这些发现为评估消毒/氧化过程中ARG的命运提供了定量框架，并支持使用qPCR作为在精心选择的情况下跟踪ARG失活的代理。

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  • 来源
    《Environmental Science & Technology》 |2019年第4期|2013-2026|共14页
  • 作者

    He Huan; Zhou Peiran; Shimabuku Kyle K.; Fang Xuzhi; Li Shu; Lee Yunho; Dodd Michael C.;

  • 作者单位

    Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA;

    Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA;

    Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA;

    Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA;

    Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA;

    GIST, Sch Earth Sci & Environm Engn, Gwangju 61005, South Korea;

    Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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