Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination

Yin Yongchao; Yan Jun; Chen Gao; Murdoch Fadime Kara; Pfisterer Nina; Loffler Frank E.

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Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination

机译：一氧化二氮是细菌还原性脱氯的有效抑制剂

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Organohalide-respiring bacteria are key players for the turnover of organohalogens. At sites impacted with chlorinated ethenes, bioremediation promotes reductive dechlorination; however, stoichiometric conversion to environmentally benign ethene is not always achieved. We demonstrate that nitrous oxide (N2O), a compound commonly present in groundwater, inhibits organohalide respiration. N2O concentrations in the low micromolar range decreased dechlorination rates and resulted in incomplete dechlorination of tetrachloroethene (PCE) in Geobacter lovleyi strain SZ and of cis-1,2-dichloroethene (cDCE) and vinyl chloride (VC) in Dehalococcoides mccartyi strain BAV1 axenic cultures. Presumably, N2O interferes with reductive dechlorination by reacting with super-reduced Co(I)-corrinoids of reductive dehalogenases, which is supported by the finding that N2O did not inhibit corrinoid- independent fumarate-to-succinate reduction in strain SZ. Kinetic analyses revealed a best fit to the noncompetitive Michaelis-Menten inhibition model and determined N2O inhibitory constants, K-1, for PCE and cDCE dechlorination of 40.8 +/- 3.8 and 21.2 +/- 3.5 mu M in strain SZ and strain BAV1, respectively. The lowest K-1, value of 9.6 +/- 0.4 mu M was determined for VC to ethene reductive dechlorination in strain BAV1, suggesting that this crucial dechlorination step for achieving detoxification is most susceptible to N2O inhibition. Groundwater N2O concentrations exceeding 100 mu M are not uncommon, especially in watersheds impacted by nitrate runoff from agricultural sources. Thus, dissolved N2O measurements can inform about cDCE and VC stalls at sites impacted with chlorinated ethenes.

机译：呼吸有机卤化物的细菌是有机卤素转换的关键因素。在受到氯化乙烯影响的场所，生物修复促进了还原性脱氯。然而，并非总是实现化学计量转化为环境友好的乙烯。我们证明了一氧化二氮（N2O），一种常见于地下水中的化合物，可抑制有机卤化物的呼吸。低微摩尔范围内的N2O浓度会降低脱氯速率，并导致loveoi格列佛菌株SZ中的四氯乙烯（PCE）和Dehalococcoides mccartyi菌株BAV1厌氧培养物中的顺式1,2-二氯乙烯（cDCE）和氯乙烯（VC）不完全脱氯。据推测，N2O通过与还原型脱卤化酶的超还原Co（I）-类皮质醇反应来干扰还原性脱氯，这得到以下发现的支持：N2O不会抑制菌株SZ中不依赖类固醇的富马酸酯对琥珀酸酯的还原。动力学分析表明，该化合物最适合非竞争性Michaelis-Menten抑制模型，并确定了SZ菌株和BAV1菌株中PCE和cDCE脱氯的N2O抑制常数K-1为40.8 +/- 3.8和21.2 +/- 3.5μM，分别。确定了BAV1菌株中VC还原乙烯进行乙烯还原脱氯的最低K-1值为9.6 +/- 0.4μM，这表明实现脱毒的关键脱氯步骤最容易受到N2O抑制作用。地下水中N2O浓度超过100微米的情况并不少见，特别是在受到农业来源硝酸盐径流影响的流域中。因此，溶解的N2O测量值可以告知受氯化乙烯影响的地点的cDCE和VC失速。

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《Environmental Science & Technology》 |2019年第2期|692-701|共10页
作者
Yin Yongchao; Yan Jun; Chen Gao; Murdoch Fadime Kara; Pfisterer Nina; Loffler Frank E.;
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作者单位

Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA|Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37996 USA|Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA;

Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA|Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Liaoning, Peoples R China;

Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37996 USA|Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN 37996 USA;

Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA|Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37996 USA|Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA;

Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA|Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37996 USA|Bielefeld Univ, Fac Biol, RNA Biol & Mol Physiol, D-101133 Bielefeld, Germany;

Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA|Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37996 USA|Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN 37996 USA|Univ Tennessee, Dept Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA|Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination

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