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首页> 外文期刊>Environmental Science & Technology >Dominant Role of Silver Ions in Silver Nanoparticle Toxicity to a Unicellular Alga: Evidence from Luminogen Imaging
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Dominant Role of Silver Ions in Silver Nanoparticle Toxicity to a Unicellular Alga: Evidence from Luminogen Imaging

机译：银离子对单细胞藻类的纳米银毒性中的主导作用：发光成像的证据。

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Despite the extensive studies on silver nanoparticle (AgNP) toxicity, the contribution of released silver ions to the toxicity still remains debatable. This study investigated the toxicity of AgNPs with different sizes (20 and 60 nm) toward a phytoplankton Euglena gracilis, with a focus on evaluating the contributions of dissolved Ag to the toxicity by combining a newly developed aggregation-induced emission luminogen (AIEgen)-based imaging technique and a traditional inductively coupled plasma mass spectrometry (ICP-MS) method. Smaller AgNPs, which dissolved much faster, exhibited greater toxicity, as evidenced by lower 50% growth inhibition concentration (EC50). However, the average Ag+ concentration at each EC50 was comparable to the EC50 of AgNO3, and similar subcellular Ag distribution patterns were observed in both AgNPs and AgNO3 exposed algae. More silver ions were internalized in algae treated with smaller AgNPs. With the application of luminogen imaging technology, we for the first time demonstrated that few Ag(I) were presented intracellularly in algae exposed to AgNPs when any released Ag in the medium was complexed by cysteine. Ag(I) was mainly distributed in cellular debris, organelles, and metal-rich granules fractions. No conclusive evidence for AgNP internalization was documented. Our results strongly suggested that Ag+ released from AgNPs extracellularly dominated the AgNP toxicity. The findings of this work provide new and useful insight into the toxicity of AgNPs in aquatic environments.
机译：尽管对银纳米颗粒（AgNP）毒性进行了广泛的研究，但释放的银离子对毒性的贡献仍然值得商bat。这项研究调查了不同大小（20和60 nm）的AgNPs对浮游植物Euglena gracilis的毒性，重点在于通过结合新开发的基于聚集诱导发射发光素（AIEgen）的方法评估溶解的Ag对毒性的贡献。成像技术和传统的电感耦合等离子体质谱（ICP-MS）方法。溶解速度更快的较小AgNPs表现出更大的毒性，这一点可通过降低50％的生长抑制浓度（EC50）来证明。但是，每个EC50的平均Ag +浓度与AgNO3的EC50相当，并且在暴露于AgNPs和AgNO3的藻类中均观察到类似的亚细胞Ag分布模式。在用较小的AgNP处理的藻类中，更多的银离子被内在化。随着发光原成像技术的应用，我们首次证明，当培养基中释放的任何半胱氨酸与半胱氨酸络合时，暴露于AgNPs的藻类中几乎没有Ag（I）出现在细胞内。 Ag（I）主要分布在细胞碎片，细胞器和富含金属的颗粒部分中。没有关于AgNP内部化的确凿证据。我们的结果强烈表明，从AgNPs释放的Ag +在细胞外占主导地位。这项工作的发现为了解AgNPs在水生环境中的毒性提供了新的有用的见解。

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  • 来源
    《Environmental Science & Technology》 |2019年第1期|494-502|共9页
  • 作者

    Zhang Luqing; Wang Wen-Xiong;

  • 作者单位

    HKUST Shenzhen Res Inst, Marine Environm Lab, Shenzhen 518057, Peoples R China|Hong Kong Univ Sci & Technol, Dept Ocean Sci, Kowloon, Clear Water Bay, Hong Kong, Peoples R China;

    HKUST Shenzhen Res Inst, Marine Environm Lab, Shenzhen 518057, Peoples R China|Hong Kong Univ Sci & Technol, Dept Ocean Sci, Kowloon, Clear Water Bay, Hong Kong, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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