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首页> 外文期刊>Environmental Science & Technology >Novel Insights into the Role of Copper in Critical Life Stages of Oysters Revealed by High-Resolution NanoSIMS Imaging
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Novel Insights into the Role of Copper in Critical Life Stages of Oysters Revealed by High-Resolution NanoSIMS Imaging

机译：高分辨率NanoSIMS成像揭示了铜在牡蛎关键生命阶段中的作用的新见解

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摘要

Copper (Cu) is a key trace element for many biological processes, but there is little available information regarding its requirements and functions in critical life stages of marine bivalves, during which dramatic morphological and physiological changes occur. In this study, the ontogeny pattern of Cu accumulation across the life history of oysters was explored for the first time and the distributions of Cu in oysters at critical life stages (pediveliger and early settled spat) were in situ mapped by nanoscale secondary ion mass spectrometry (NanoSIMS) with high lateral resolution. We first demonstrated that the late pelagic stage to early settled stage was the critical stage requiring Cu during oyster development. NanoSIMS imaging revealed a significant elevation of intercellular Cu levels along with mitochondrial calcium overload and obvious structural degradation of mitochondria in velar cells at the pediveliger stage, implying the possible role of Cu in cell apoptosis of the velum during larval metamorphosis. Furthermore, an obvious enrichment of Cu together with calcium was observed in the nucleus and mitochondria of ciliated cells of gill tissue at the early settled stage. Their accumulation in the gill cells was significantly higher than that in the juveniles, indicating the potential role of Cu in sustaining the fast growth of the gill filament and the concomitant acceleration of energy metabolism during early benthic development. Our findings offer new insights into the understanding of the interactions between trace metals and marine bivalves.
机译：铜（Cu）是许多生物过程中的关键微量元素，但是在海洋双壳类动物的关键生命阶段中，其形态和生理发生了巨大变化，因此关于其需求和功能的可用信息很少。在这项研究中，首次探索了牡蛎整个生命历程中铜积累的个体发育模式，并通过纳米级二次离子质谱法原位绘制了牡蛎在关键生命阶段（定性配子和早期沉降的飞沫）中的铜分布（NanoSIMS）具有高横向分辨率。我们首先证明了上层晚期到定居早期是牡蛎发育过程中需要铜的关键时期。 NanoSIMS成像显示在除草剂阶段，绒毛膜细胞中细胞间铜水平显着升高，线粒体钙超载，线粒体结构发生明显降解，这暗示着铜在幼虫变态期间可能在膜细胞凋亡中发挥作用。此外，在早期沉降阶段，在tissue组织的纤毛细胞的核和线粒体中观察到了铜和钙的明显富集。它们在the细胞中的积累显着高于幼鱼，表明铜在维持底栖动物早期发育过程中在维持filament丝快速生长和伴随能量代谢加速中的潜在作用。我们的发现为了解痕量金属与海洋双壳类动物之间的相互作用提供了新的见解。

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  • 来源
    《Environmental Science & Technology》 |2019年第24期|14724-14733|共10页
  • 作者

    Weng Nanyan; Jiang Haibo; Wang Wen-Xiong;

  • 作者单位

    HKUST Shenzhen Res Inst Shenzhen Key Lab Edible & Med Bioresources Shenzhen 518057 Guangdong Peoples R China|HKUST Dept Ocean Sci Kowloon Clearwater Bay Hong Kong 999077 Peoples R China|HKUST Hong Kong Branch Southern Marine Sci & Engn Guangdong Lab Guangzho Kowloon Clearwater Bay Hong Kong 999077 Peoples R China;

    Univ Western Australia Sch Mol Sci Perth WA 6009 Australia|Univ Western Australia Ctr Microscopy Charaterisat & Anal Perth WA 6009 Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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