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首页> 外文期刊>Environmental Science & Technology >Size-Resolved Single-Particle Fluorescence Spectrometer for Real- Time Analysis of Bioaerosols: Laboratory Evaluation and Atmospheric Measurements
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Size-Resolved Single-Particle Fluorescence Spectrometer for Real- Time Analysis of Bioaerosols: Laboratory Evaluation and Atmospheric Measurements

机译：用于生物气溶胶实时分析的尺寸分辨单颗粒荧光光谱仪：实验室评估和大气测量

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摘要

Characteristic particle size, fluorescence intensity, and fluorescence spectra are important features to detect and categorize bioaerosols. A prototype size-resolved single-particle fluorescence spectrometer (S2FS) was developed to simultaneously measure aerodynamic diameters and fluorescence spectra. Emission spectra are dispersed in 512 channels from 370 to 610 nm, where a major portion of biological fluorescence emission occurs. The S2FS consists of an aerodynamic particle sizer and a fluorescence spectrometer with a 355 nm laser excitation source and an intensified charge-coupled device as the detector. Highly fluorescent particles, such as Ambrosia artemisiifolia pollen and Olea europaea pollen, can be distinguished by the S2FS on a single-particle level. For weakly fluorescent particles, fluorescence spectra can only be obtained by averaging multiple particles (between 100 and 3000) of the same kind. Preliminary ambient measurements in Mainz (Germany, central Europe) show that an emission peak at similar to 440 nm was frequently observed for fluorescent fine particles (0.5-1 mu m). Fluorescent fine particles accounted for 2.8% on average based on the number fraction in the fine mode. Fluorescent coarse particles (>1 mu m) accounted for 8.9% on average based on the number fraction, with strongest occurrence observed during a thunderstorm and in the morning.
机译：特征粒径，荧光强度和荧光光谱是检测和分类生物气溶胶的重要特征。开发了原型尺寸分辨单粒子荧光光谱仪（S2FS），以同时测量空气动力学直径和荧光光谱。发射光谱分散在370至610 nm的512个通道中，其中大部分发生了生物荧光发射。 S2FS由空气动力学粒度仪和带有355 nm激光激发源和增强型电荷耦合器件作为检测器的荧光光谱仪组成。 S2FS可以在单颗粒水平上区分高度荧光的颗粒，例如青蒿花粉和油橄榄花粉。对于弱荧光粒子，只能通过平均相同种类的多个粒子（100到3000之间）来获得荧光光谱。在美因茨（德国，中欧）进行的初步环境测量表明，对于荧光细颗粒（0.5-1μm）经常观察到类似于440 nm的发射峰。基于精细模式下的数量分数，荧光微粒平均占2.8％。荧光粗颗粒（> 1微米）平均占总数的8.9％，在雷暴期间和早晨观察到的现象最大。

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  • 来源
    《Environmental Science & Technology》 |2019年第22期|13257-13264|共8页
  • 作者

    Zhang Minghui; Klimach Thomas; Ma Nan; Koenemann Tobias; Poehlker Christopher; Wang Zhibin; Kuhn Uwe; Scheck Nelli; Poeschl Ulrich; Su Hang; Cheng Yafang;

  • 作者单位

    Max Planck Inst Chem Multiphase Chem Dept D-55128 Mainz Germany;

    Jinan Univ Inst Environm & Climate Res ECI Ctr Air Pollut & Climate Change Res Guangzhou 511443 Guangdong Peoples R China;

    Max Planck Inst Chem Particle Chem Dept D-55128 Mainz Germany|Zhejiang Univ Coll Environm & Resource Sci Res Ctr Air Pollut & Hlth Hangzhou 310058 Zhejiang Peoples R China;

    Max Planck Inst Chem Particle Chem Dept D-55128 Mainz Germany;

    Max Planck Inst Chem Multiphase Chem Dept D-55128 Mainz Germany|Max Planck Inst Chem Minerva Res Grp D-55128 Mainz Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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