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首页> 外文期刊>Environmental Science & Technology >Connecting the Oxidative Potential of Secondary Organic Aerosols with Reactive Oxygen Species in Exposed Lung Cells
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Connecting the Oxidative Potential of Secondary Organic Aerosols with Reactive Oxygen Species in Exposed Lung Cells

机译：在暴露的肺细胞中将次要有机气溶胶的氧化电位与活性氧联系起来

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It has been hypothesized that the cytotoxicity of secondary organic aerosols (SOA) is mediated through the formation of reactive oxygen species (ROS) in the exposed cells. Here, lung epithelial cells (A549) residing at the air-liquid interface were exposed to proxies of anthropogenic and biogenic SOA that were photochemically aged under varying nitrogen oxide (NOx) concentrations in an oxidation flow reactor. The total organic peroxides and ROS radical content in the SOA were quantified by the iodometric spectrophotometric method and by continuous-wave electron paramagnetic resonance. The effect of the exposure was evaluated by measuring cell viability and cellular ROS production following the exposure. The results demonstrate that SOA that aged in the absence of NOx contained more ROS than fresh SOA and were more toxic toward the cells, while varying NOx conditions had no significant influence on levels of the ROS content in fresh SOA and their toxicity. Analysis of ROS in the exposed cells using flow cytometry showed a similar trend with the total ROS content in the SOA. This study provides a first and direct observation of such association.
机译：假设次级有机气溶胶（SOA）的细胞毒性是通过在暴露的细胞中形成活性氧（ROS）来介导的。在此，将位于气液界面的肺上皮细胞（A549）暴露于人为和生物源SOA的代理下，这些代理在氧化流反应器中以不同的氮氧化物（NOx）浓度进行了光化学老化。通过碘量分光光度法和连续波电子顺磁共振对SOA中的总有机过氧化物和ROS自由基含量进行定量。通过测量暴露后的细胞活力和细胞ROS产生来评估暴露的效果。结果表明，在没有NOx的情况下老化的SOA比新鲜SOA含有更多的ROS，并且对细胞更具毒性，而不同的NOx条件对新鲜SOA中ROS含量及其毒性没有显着影响。使用流式细胞仪分析暴露细胞中的ROS，发现与SOA中总ROS含量相似的趋势。这项研究提供了这种关联的第一个直接观察。

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