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Reproduction Stage Differentiates the Time-Course Regulation of Metabolites in Daphnia magna

机译：繁殖阶段区分大型蚤的代谢产物的时间过程调节

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摘要

Daphnia magna is a keystone indicator zooplankton used in environmental quality assessments. Comparative metabolomics, which contrasts small biomolecular regulations under different conditions, has emerged as a sensitive and informative investigation tool for the assessment of environmental stressors on D. magna. Baseline metabolomic variation is likely impacted by the asexual reproduction cycle of D. magna; however, the relationship between metabolite concentration regulation and reproduction cycle has not been investigated. This study investigated the time-course regulation of 51 metabolites during different reproduction stages to determine how the reproduction cycle controlled the metabolite profile of D. magna. Two-way analysis of variance (ANOVA) results reveal that most metabolites show significantly differentiated concentrations by individual or a combination of reproduction stages and sampling time. On the basis of the partial least-squares-discriminant analysis (PLS-DA) and ANOVA-simultaneous component analysis (ANOVA-SCA), stages 2 and 3 of reproduction show similarity in metabolite abundance regulation compared to stage 1. Metabolites were classified as being either dependent or independent of reproduction in the pairwise concentration correlation based on Pearson correlation coefficients. This study observed that the D. magna reproduction stage is an important consideration and potential variable and should be considered carefully when conducting metabolomic experiments using D. magna.
机译：大型蚤（Daphnia magna）是用于环境质量评估的重要指标浮游动物。比较代谢组学已在不同条件下与小生物分子法规进行了对比，它已成为一种敏感而信息丰富的调查工具，可用于评估D. magna的环境应激源。基线代谢组学变异可能受到D. magna无性繁殖周期的影响。然而，尚未研究代谢物浓度调节与繁殖周期之间的关系。这项研究调查了51个代谢产物在不同繁殖阶段的时程调节，以确定繁殖周期如何控制D. magna的代谢产物。双向方差分析（ANOVA）结果表明，大多数代谢物显示出的个体或繁殖阶段和采样时间组合显着不同的浓度。根据偏最小二乘判别分析（PLS-DA）和ANOVA同时成分分析（ANOVA-SCA），繁殖的第2阶段和第3阶段与第1阶段相比在代谢物丰度调节方面显示出相似性。在基于Pearson相关系数的成对浓度相关中与复制无关或无关。这项研究观察到，D。magna的繁殖阶段是一个重要的考虑因素和潜在变量，在使用D. magna进行代谢组学实验时应仔细考虑。

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  • 来源
    《Environmental Science & Technology》 |2019年第21期|12764-12773|共10页
  • 作者

    Jeong Tae-Yong; Simpson Myrna J.;

  • 作者单位

    Univ Toronto Scarborough Dept Phys & Environm Sci 1265 Mil Trail Toronto ON M1C 1A4 Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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