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首页> 外文期刊>Environmental Science & Technology >Particle Size Distribution of Environmental DNA from the Nuclei of Marine Fish
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Particle Size Distribution of Environmental DNA from the Nuclei of Marine Fish

机译：海鱼细胞核中环境DNA的粒径分布

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摘要

Environmental DNA (eDNA) analyses have enabled a more efficient surveillance of species distribution and composition than conventional methods. However, the characteristics and dynamics of eDNA (e.g., origin, state, transport, and fate) remain unknown. This is especially limited for the eDNA derived from nuclei (nu-eDNA), which has recently been used in eDNA analyses. Here, we compared the particle size distribution (PSD) of nu-eDNA from Japanese Jack Mackerel (Trachurus japonicus) with that of mt-eDNA (eDNA derived from mitochondria) reported in previous studies. We repeatedly sampled rearing water from the tanks under multiple temperatures and fish biomass levels, and quantified the copy numbers of size-fractioned nu-eDNA. We found that the concentration of nu-eDNA was higher than that of mt-eDNA at 3-10 mu m size fraction. Moreover, at the 0.8-3 mu m and 0.4-0.8 mu m size fractions, eDNA concentrations of both types increased with higher temperature and their degradation tended to be suppressed. These results imply that the production of eDNA from large to small size fractions could buffer the degradation of small-sized eDNA, which could improve its persistence in water. Our findings will contribute to refine the difference between nu- and mt-eDNA properties, and assist eDNA analyses as an efficient tool for the conservation of aquatic species.
机译：与传统方法相比，环境DNA（eDNA）分析使物种分布和组成的监测更加有效。但是，eDNA的特征和动力学（例如起源，状态，运输和命运）仍然未知。对于最近已用于eDNA分析的源自核的eDNA（nu-eDNA）尤其如此。在这里，我们比较了先前研究中报道的来自日本Jack Mackerel（Trachurus japonicus）的nu-eDNA的粒径分布（PSD）和mt-eDNA（来自线粒体的eDNA）的粒径分布。我们在多个温度和鱼类生物量水平下，从水箱中反复取样饲养水，并量化了大小分级的nu-eDNA的拷贝数。我们发现nu-eDNA的浓度在3-10μm的范围内高于mt-eDNA。此外，在0.8-3μm和0.4-0.8μm大小的级分下，两种类型的eDNA浓度都随着温度的升高而增加，并且倾向于抑制其降解。这些结果表明，从大到小级分的eDNA的产生可以缓冲小尺寸eDNA的降解，从而可以改善其在水中的持久性。我们的发现将有助于改善nu-和mt-eDNA特性之间的差异，并有助于eDNA分析作为保护水生物种的有效工具。

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  • 来源
    《Environmental Science & Technology》 |2019年第16期|9947-9956|共10页
  • 作者

    Jo Toshiaki; Arimoto Mio; Murakami Hiroaki; Masuda Reiji; Minamoto Toshifumi;

  • 作者单位

    Kobe Univ Grad Sch Human Dev & Environm Nada Ku 3-11 Tsurukabuto Kobe Hyogo 6578501 Japan|Japan Soc Promot Sci Chiyoda Ku 5-3-1 Kojimachi Tokyo 1020083 Japan;

    Kobe Univ Fac Human Dev Nada Ku 3-11 Tsurukabuto Kobe Hyogo 6578501 Japan;

    Kyoto Univ Field Sci Educ & Res Ctr Maizuru Fisheries Res Stn Maizuru Kyoto 6250086 Japan;

    Kobe Univ Grad Sch Human Dev & Environm Nada Ku 3-11 Tsurukabuto Kobe Hyogo 6578501 Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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