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首页> 外文期刊>Environmental Science & Technology >Structure-Directed Screening and Analysis of Thyroid-Disrupting Chemicals Targeting Transthyretin Based on Molecular Recognition and Chromatographic Separation
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Structure-Directed Screening and Analysis of Thyroid-Disrupting Chemicals Targeting Transthyretin Based on Molecular Recognition and Chromatographic Separation

机译：基于分子识别和色谱分离的靶向运甲状腺素蛋白的破坏甲状腺的化学物质的结构定向筛选和分析

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摘要

Exposure to thyroid-disrupting chemicals (TDCs) poses a great threat to human health. However, the screening and analysis of TDCs in environmental samples remain a tough work. In this study, we reported a structure-directed strategy for analyzing TDCs targeting transthyretin (TTR) based on molecular imprinting and chromatographic separation. The imprinted composites were prepared using L-thyroxine (T4) as a template and a tryptophan-like monomer screened from the amino acid library. The imprinted composites exhibited an adsorption capacity of 22.2 μmol g~(-1) for T4 and an imprinting factor of 2.1. Chromatographic testing was then conducted among 72 chemicals using the imprinted composites-packed column. High retention factors were observed for chemicals that were structurally similar to T4. The chromatographic results were compared with a data set of 45 chemicals with known activities toward TTR. The results suggested that chemicals can be distinguished as TTR binders and nonbinders by retention factors, with a predictive accuracy of more than 90%. Moreover, the retention factors of chemicals were highly correlated with the reported relative potencies obtained from TTR assays. Thus, screening of TTR-binding chemicals can be realized through this simple chromatographic method. The imprinted composites were applied for target analysis and nontarget analysis of TTR-binding chemicals in dust samples. Three new TTR binders were successfully identified and verified by this method. The combination of molecular imprinting and chromatography opens up a new approach for screening TDCs targeting TTR.
机译：接触破坏甲状腺的化学物质（TDC）对人类健康构成了巨大威胁。但是，对环境样品中TDC的筛选和分析仍然是一项艰巨的工作。在这项研究中，我们报告了一种基于分子印迹和色谱分离的靶向T甲状腺素（TTR）的TDC分析的结构导向策略。使用L-甲状腺素（T4）作为模板，并从氨基酸库中筛选出色氨酸样单体，制备了印迹复合物。印迹复合材料对T4的吸附容量为22.2μmolg〜（-1），印迹因子为2.1。然后使用压印的复合填料色谱柱对72种化学物质进行色谱测试。对于与T4结构相似的化学药品，观察到了较高的保留因子。色谱结果与45种已知对TTR有活性的化学物质的数据集进行了比较。结果表明，可以通过保留因子将化学物质区分为TTR粘合剂和非粘合剂，其预测精度超过90％。此外，化学物质的保留因子与报告的从TTR分析获得的相对效力高度相关。因此，可以通过这种简单的色谱方法来筛选TTR结合化学品。印迹复合材料用于灰尘样品中TTR结合化学物质的目标分析和非目标分析。通过此方法成功鉴定并验证了三种新型TTR粘合剂。分子印迹和色谱的结合为筛选靶向TTR的TDC开辟了一种新方法。

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  • 来源
    《Environmental Science & Technology》 |2020年第9期|5437-5445|共9页
  • 作者

    Kai Huang; Xiu Wang; Hongxing Zhang; Lingshuai Zeng; Xiu Zhang; Bingmao Wang; Yikai Zhou; Tao Jing;

  • 作者单位

    State Key Laboratory of Environment Health (Incubation) Key Laboratory of Environment and Health Ministry of Education Key Laboratory of Environment and Health (Wuhan) Ministry of Environmental Protection School of Public Health Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 P. R. China;

    State Key Laboratory of Environment Health (Incubation) Key Laboratory of Environment and Health Ministry of Education Key Laboratory of Environment and Health (Wuhan) Ministry of Environmental Protection School of Public Health Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 P. R. China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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