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首页> 外文期刊>Environmental Science & Technology >Single-Cell RNA Sequencing Reveals Size-Dependent Effects of Polystyrene Microplastics on Immune and Secretory Cell Populations from Zebrafish Intestines
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Single-Cell RNA Sequencing Reveals Size-Dependent Effects of Polystyrene Microplastics on Immune and Secretory Cell Populations from Zebrafish Intestines

机译：单细胞RNA测序揭示了斑马鱼肠的免疫和分泌细胞群体的聚苯乙烯微塑料的大小依赖的影响。

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摘要

Microplastics (MPs) as widespread contamination pose a high risk for aquatic organisms. However, the current understanding of MP toxicity is based on cell population-averaged measurements. Our aim was to gain a comprehensive understanding of the size-dependent effects of polystyrene MPs (PS-MPs) on intestinal cell populations in zebrafish and characterize the interplay of MPs, intestinal cells, and intestinal microbiota. Here, we used single-cell RNA sequencing to determine the transcriptome heterogeneity of 12 000 intestinal cells obtained from zebrafish exposed to 100 nm, 5 μm, and 200 μm PS-MPs for 21 days. Eight intestinal cell populations were identified. Combined with changes in intestinal microbiota, our findings highlight a previously unrecognized end point that all three sizes of PS-MPs induced dysfunction of intestinal immune cells (including effects on phagosomes and the regulation of immune system processes) and increased the abundance of pathogenic bacteria. However, only 100 nm PS-MPs altered the expression of genes related to phagocyte-produced reactive oxygen species (ROS) generation and increased mucus secretion by secretory cells. Microsize PS-MPs specifically changed the lysosome (5 μm) and cell surface receptor signaling (200 μm) processes of the macrophages. Our findings pinpoint to cell-specific and size-dependent responses to PS-MPs in fish intestine, which can provide a reference for future study directions.
机译：作为广泛污染的微塑料（MPs）对水生生物构成高风险。但是，目前对MP毒性的了解是基于细胞群体平均测量值。我们的目的是全面了解斑马鱼中聚苯乙烯MP（PS-MP）对肠道细胞种群的大小依赖性作用，并表征MP，肠道细胞和肠道菌群之间的相互作用。在这里，我们使用单细胞RNA测序来确定从暴露在100 nm，5μm和200μmPS-MP中21天的斑马鱼中获得的12000个肠道细胞的转录组异质性。确定了八个肠道细胞群。结合肠道菌群的变化，我们的发现突出了以前无法识别的终点，即所有三种大小的PS-MP均引起肠道免疫细胞功能障碍（包括对吞噬体的影响和免疫系统过程的调节），并增加了致病细菌的丰度。但是，只有100 nm PS-MP改变了与吞噬细胞产生的活性氧（ROS）产生相关的基因表达，并增加了分泌细胞的粘液分泌。微米级PS-MP特异性改变了巨噬细胞的溶酶体（5μm）和细胞表面受体信号传导（200μm）过程。我们的发现指出了鱼肠对PS-MP的细胞特异性和大小依赖性反应，这可以为将来的研究方向提供参考。

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  • 来源
    《Environmental Science & Technology》 |2020年第6期|3417-3427|共11页
  • 作者

    Weiqing Gu; Su Liu; Ling Chen; Yuxuan Liu; Cheng Gu; Hong-qiang Ren; Bing Wu;

  • 作者单位

    State Key laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing Jiangsu 210023 P.R. China;

    State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing jiangsu 210023 P.R. China Department of Environmental Science School of Engineering China Pharmaceutical University Nanjing Jiangsu 211198 P.R. China;

    Stare Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing Jiangsu 210023 P.R. China;

    College of Environment Hohai University Nanjing Jiangsu 210098 P.R. China;

    State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing Jiangsu 210023 P.R. China;

    State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing Jiangsu 210023 P.R. China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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