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首页> 外文期刊>Environmental Science & Technology >Proteolysis of Iron Oxide-Associated Bovine Serum Albumin
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Proteolysis of Iron Oxide-Associated Bovine Serum Albumin

机译：氧化铁相关的牛血清白蛋白的蛋白水解

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摘要

Proteins are a substantial nitrogen source in soils provided that they can be hydrolyzed into bioavailable small peptides or amino acids. However, the strong associations between proteins and soil minerals restrict such proteolytic reactions. This study focused on how an extracellular fungal protease (Rhizopus sp.) hydrolyzed iron oxide-associated bovine serum albumin (BSA) and the factors that affected the proteolysis. We combined batch experiments with size-exclusion and reversed phase liquid chromatography and in situ infrared spectroscopic measurements to monitor the generation of proteolytic products in solution as well as the real-time changes of the adsorbed BSA during 24 h. Results showed that protease hydrolyzed the iron oxide-associated BSA directly at the surface without an initial desorption of BSA. Concurrently, the protease was adsorbed to vacant surface sites at the iron oxides, which significantly slowed down the rate of proteolysis. This inhibiting effect was counteracted by the presence of preadsorbed phosphate or by increasing the BSA coverage, which prevented protease adsorption. Fast initial rates of iron oxide-associated BSA proteolysis, comparable to proteolysis of BSA in solution, and very slow rates at prolonged proteolysis suggest a large variability in mineral-associated proteins as a nitrogen source in soils and that only a fraction of the protein is bioavailable.
机译：蛋白质是土壤中的重要氮源，只要可以将其水解为可生物利用的小肽或氨基酸即可。但是，蛋白质和土壤矿物质之间的强关联限制了这种蛋白水解反应。这项研究的重点是细胞外真菌蛋白酶（Rhizopus sp。）如何水解氧化铁相关的牛血清白蛋白（BSA）以及影响蛋白水解的因素。我们将批量实验与尺寸排阻和反相液相色谱以及原位红外光谱测量相结合，以监测溶液中蛋白水解产物的产生以及24小时内吸附的BSA的实时变化。结果表明，蛋白酶直接在表面水解了与氧化铁相关的BSA，而没有BSA的初始解吸。同时，蛋白酶被吸附到氧化铁的空位表面，这大大减慢了蛋白水解的速度。这种抑制作用被预吸附的磷酸盐的存在或增加了BSA的覆盖率所抵消，这阻止了蛋白酶的吸附。与溶液中BSA的蛋白水解相当的氧化铁相关BSA蛋白质水解的初始速率快，而在长时间的蛋白水解中非常慢的速率表明矿物相关蛋白质作为土壤中的氮源存在很大差异，并且只有一部分蛋白质是生物利用度。

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  • 来源
    《Environmental Science & Technology》 |2020年第8期|5121-5130|共10页
  • 作者

    Zhaomo Tian; Tao Wang; Anders Tunlid; Per Persson;

  • 作者单位

    Department of Biology Microbial Ecology Group and Centre for Environmental and Climate Research (CEC) Lund University SE-223 62 Lund Sweden;

    Department of Biology Microbial Ecology Group Lund University SE-223 62 Lund Sweden;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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