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首页> 外文期刊>Environmental Science & Technology >Developmental Effects and Estrogenicity of Bisphenol A Alternatives in a Zebrafish Embryo Model
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Developmental Effects and Estrogenicity of Bisphenol A Alternatives in a Zebrafish Embryo Model

机译：双酚的发育效应和雌激素Zebrafish胚胎模型中的替代品

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摘要

In order to understand the negative effects of bisphenol A (BPA) alternatives comprehensively, zebrafish embryos were used to assess the lethality, developmental effects, and estrogenic activity of bisphenol analogues. The in silico estrogenic activities of bisphenol analogues were assayed by binding simulation. According to our results, the lethality of bisphenol analogues decreased in order of bisphenol AF (BPAF) BPA bisphenol F (BPF) bisphenol S (BPS). BPAF and BPF induced significant effects on zebrafish embryos, including decreased heart rate, hatching inhibition, and teratogenic effects. The binding potentials of bisphenol analogues toward zebrafish ERs (zfERS) decreased in the following order: BPAF BPA BPF BPS. Among the three subtypes of zfERs, zfER beta 2 showed the highest binding activity toward the bisphenols, followed by zfER alpha and zfER beta 1. In vivo estrogenic activity tests showed that BPAF, BPA, and BPF significantly enhanced the protein levels of ER alpha along with the mRNA levels of esrl, esr2a, esr2b, and vtgl in zebrafish embryos. Esr2b showed the strongest response to BPAF and BPA exposure among the three esrs. In contrast, BPS did not significantly regulate ER protein level or ER transcription. In conclusion, BPAF showed the highest lethality, developmental effects, and estrogenic activity (both in silico and in vivo) followed by BPA and BPF. BPS showed the weakest toxicity and estrogenic activity. zfER beta 2 might act as the main target among the three ER subtypes of zebrafish after exposure to BPAF and BPA.
机译：为了理解双酚A（BPA）替代方案的负面影响，Zebrafish胚胎用于评估双酚类似物的致死态，发育效果和雌激素活性。通过结合模拟测定双酚类似物的硅雌激素活性。根据我们的结果，双酚AF（BPAF）> BPA>双酚F（BPF）>双酚S（BPS）下降的双酚类似物的致死性降低。 BPAF和BPF对斑马鱼胚胎产生显着影响，包括降低心率，孵化抑制和致畸作用。双酚类似物朝向斑马鱼的结合电位以下列顺序降低：BPAF> BPA> BPF> BPS。在Zfers的三个亚型中，Zferβ2显示了双酚的最高结合活性，然后是Zferα和Zferβ11。在体内雌激素的活性试验中，BPAF，BPA和BPF显着提高了ERα的蛋白质水平随着ESRL，ESR2A，ESR2B和斑马鱼胚胎中VTGL的mRNA水平。 ESR2B表现出对BPAF和BPA暴露的最强烈反应。相比之下，BPS没有显着调节ER蛋白质水平或ER转录。总之，BPAF显示出最高的致死性，发育效果和雌激素活性（硅藻和体内），然后是BPA和BPF。 BPS显示出最弱的毒性和雌激素活性。 ZFER BETA 2可能在接触BPAF和BPA后作为斑马鱼的三个ER亚型的主要目标。

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  • 来源
    《Environmental Science & Technology》 |2018年第5期|3222-3231|共10页
  • 作者

    Mu Xiyan; Huang Ying; Li Xuxing; Lei Yunlei; Teng Miaomiao; Li Xuefeng; Wang Chengju; Li Yingren;

  • 作者单位

    Chinese Acad Fishery Sci Fishery Resource & Environm Res Ctr Beijing 100141 Peoples R China;

    Chinese Acad Fishery Sci Fishery Resource & Environm Res Ctr Beijing 100141 Peoples R China;

    Chinese Acad Fishery Sci Fishery Resource & Environm Res Ctr Beijing 100141 Peoples R China;

    Chinese Acad Fishery Sci Fishery Resource & Environm Res Ctr Beijing 100141 Peoples R China;

    China Agr Univ Coll Sci Beijing 100193 Peoples R China;

    China Agr Univ Coll Sci Beijing 100193 Peoples R China;

    China Agr Univ Coll Sci Beijing 100193 Peoples R China;

    Chinese Acad Fishery Sci Fishery Resource & Environm Res Ctr Beijing 100141 Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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