...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Water Disinfection Byproducts Increase Natural Transformation Rates of Environmental DNA in Acinetobacter baylyi ADP1
【24h】

Water Disinfection Byproducts Increase Natural Transformation Rates of Environmental DNA in Acinetobacter baylyi ADP1

机译：水消毒副产品增加了百叶腺素ADP1中的环境DNA的自然转化率

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The process of natural transformation allows for the stable uptake, integration, and functional expression of extracellular DNA. This mechanism of horizontal gene transfer has been widely linked to the acquisition of antibiotic resistance and virulence factors. Here, we demonstrate that bromoacetic acid (BAA) a regulated drinking water disinfection byproduct (DBP) can stimulate natural transformation rates in the model organism Acinetobacter baylyi ADP1. We demonstrate that transformation stimulation in response to BAA is concentration-dependent and is linked to the ability of this compound to generate DNA damage via oxidative stress. In presence of BAA, transcription of recA was upregulated 20-40% compared to the nontreated controls, indicating that this component of the DNA damage response could be associated with the increase in transformation. Other genes associated with DNA translocation across the cytoplasmic membrane (i.e., pilX, comA) did not exhibit increased transcription in the presence of BAA, indicating that the enhancement of transformation is not associated with increased translocation rates of environmental DNA. Overall, these results lead us to speculate that elevated recA transcription levels could lead to increased integration rates of foreign DNA within the recipient cell during DNA repair. Lastly, we show that an artificial DBP cocktail simulating the environmental concentrations of five water DBP classes stimulates natural transformation by almost 2-fold. The results of this study suggest that mutagens like DBPs may play an important role in enhancing the fixation rates of extracellular DNA in the environmental metagenome.
机译：天然转化的过程允许细胞外DNA的稳定摄取，集成和功能表达。这种水平基因转移机制与获取抗生素抗性和毒力因子已被广泛相关。在此，我们证明了溴乙酸（BAA）调节饮用水消毒副产物（DBP）可以刺激模型生物传染病的自然转化率百叶腺ADP1。我们证明响应BAA的转化刺激是浓度依赖性的，并且与该化合物通过氧化应激产生DNA损伤的能力相关。在BAA存在下，与非处理对照相比，RECA的转录将上调20-40％，表明DNA损伤反应的这种组分可能与转化的增加有关。与细胞质膜（即Pilx，Coma）的DNA易位相关的其他基因在BAA存在下没有表现出增加的转录，表明转化的增强与环境DNA的易位率增加无关。总的来说，这些结果导致我们推测，在DNA修复期间，升高的REGA转录水平可能导致受体细胞内外来DNA的整合率增加。最后，我们表明，模拟五种水DBP类环境浓度的人工DBP鸡尾酒刺激了近2倍的自然变换。该研究的结果表明，如Dbps这样的诱变剂可能在提高环境梅塔群中的细胞外DNA的固定率方面发挥重要作用。

著录项

  • 来源
    《Environmental Science & Technology》 |2019年第11期|6520-6528|共9页
  • 作者

    Mantilla-Calderon David; Plewa Michael J.; Michoud Gregoire; Fodelianakis Stelios; Daffonchio Daniele; Hong Pei-Ying;

  • 作者单位

    KAUST Div Biol & Environm Sci & Engn Water Desalinat & Reuse Ctr Thuwal 239556900 Saudi Arabia;

    Univ Illinois Dept Crop Sci Urbana IL 61801 USA|Univ Illinois Safe Global Water Inst Urbana IL 61801 USA;

    KAUST Div Biol & Environm Sci & Engn Thuwal 239556900 Saudi Arabia;

    KAUST Div Biol & Environm Sci & Engn Thuwal 239556900 Saudi Arabia;

    KAUST Div Biol & Environm Sci & Engn Thuwal 239556900 Saudi Arabia;

    KAUST Div Biol & Environm Sci & Engn Water Desalinat & Reuse Ctr Thuwal 239556900 Saudi Arabia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号