...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Determination of Saccharides in Environments Using a Sulfuric Acid-Fluorescence Approach
【24h】

Determination of Saccharides in Environments Using a Sulfuric Acid-Fluorescence Approach

机译：使用硫酸 - 荧光方法测定环境中的糖类

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Accurate, rapid, and reliable quantification of saccharides is essential for understanding their behaviors and roles in environmental processes. However, the conventional colorimetric method for saccharide quantification fails to discriminate between fructose and glucose, resulting in the misestimation of total saccharides. To solve this problem, a fluorescence approach, that is, parallel factor framework-linear regression analysis, was developed in this work to quantify the specific fluorescence signatures of the fluorescent products generated from the reaction between saccharides and sulfuric acid. The fluorescent derivatives of saccharides were recognized and the simultaneous quantification of glucose and fructose was achieved with a detection limit of 2.9 μg/mL and 1.3 μg/mL, respectively. Furthermore, 200 μg/mL of the treated sorbitol and gluconic acid only, respectively, equaled to 6 μg/mL and 3 μg/mL of the treated glucose, indicating their negligible interference for the saccharide quantification using this method. In addition, the feasibility and robustness of this method in environmental applications were validated with the recovery tests using spiked real water samples. This fluorescence-based approach offers a new tool to monitor saccharides in complex environments.
机译：糖类的准确，快速和可靠的量化对于了解环境过程中的行为和角色至关重要。然而，用于糖类定量的常规比色法未能区分果糖和葡萄糖，导致总糖的折射率。为了解决该问题，在该作品中开发了一种荧光方法，即平行因子框架 - 线性回归分析，以量化糖类和硫酸之间的反应产生的荧光产品的特定荧光签名。识别糖的荧光衍生物，并分别以2.9μg/ ml和1.3μg/ ml的检出限成同时定量葡萄糖和果糖。此外，仅20μg/ mL处理的山梨糖醇和葡萄糖醇，仅等于6μg/ ml和3μg/ ml处理的葡萄糖，表明使用该方法可忽略不计的糖定量干扰。此外，使用尖刺的真正水样的恢复试验验证了这种方法在环境应用中的可行性和鲁棒性。这种基于荧光的方法提供了一种在复杂环境中监测糖类的新工具。

著录项

  • 来源
    《Environmental Science & Technology》 |2020年第11期|6632-6638|共7页
  • 作者

    Chen Qian; Wei Chen; Bo Gong; Han-Qing Yu;

  • 作者单位

    CAS Key Laboratory of Urban Pollutant Conversion Department of Applied Chemistry University of Science and Technology of China Hefei 230026 China;

    CAS Key Laboratory of Urban Pollutant Conversion Department of Applied Chemistry University of Science and Technology of China Hefei 230026 China School of Metallurgy and Environment Central South University Changsha 410083 China;

    CAS Key Laboratory of Urban Pollutant Conversion Department of Applied Chemistry University of Science and Technology of China Hefei 230026 China;

    CAS Key Laboratory of Urban Pollutant Conversion Department of Applied Chemistry University of Science and Technology of China Hefei 230026 China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号