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首页> 外文期刊>Environmental Science & Technology >Biodegradation of Polystyrene by Pseudomonas sp. Isolated from the Gut of Superworms (Larvae of Zophobas atratus)
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Biodegradation of Polystyrene by Pseudomonas sp. Isolated from the Gut of Superworms (Larvae of Zophobas atratus)

机译:PSEUDOUDOUNAS SP的聚苯乙烯生物降解。与肠道的肠道(Zophobas Atratus的幼虫)隔离

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摘要

Recently, various attempts have been made to solve plastic waste problems, such as development of biodegradation without producing pollution. Polystyrene (PS) is the fifth most used plastic in many industries; therefore, degrading PS becomes a critical global issue. Here, we reported Pseudomonas aeruginosa strain DSM 50071, initially isolated from the gut of the superworms, Zophobas atratus, and the PS degradation by Pseudomonas sp. DSM 50071. We examined PS degradation using electronic microscopy and measured changes in atomic composition and contact angles with water droplets on the PS surface that represents a chemical change from hydrophobicity to hydrophilicity. We have further examined chemical structural changes using X-ray photoelectron spectroscopy, Fourier-transform-infrared spectroscopy, and nuclear magnetic resonance (NMR) to confirm the formation of carbonyl groups (C=O) in the oxidation pathway during PS biodegradation. In reverse transcription quantitative polymerase chain reaction analysis, the gene expression level of serine hydrolase (SH) in Pseudomonas sp. DSM 50071 was highly increased during PS degradation, and the enzyme-mediated biodegradation of PS was further confirmed by the SH inhibitor treatment test. Thus, the significance of these findings goes beyond the discovery of a novel function of Pseudomonas sp. DSM 50071 in the gut of superworms, highlighting a potential solution for PS biodegradation.
机译:最近,已经制定了各种尝试来解决塑料废物问题,例如生物降解的发展而不产生污染。聚苯乙烯(PS)是许多行业中最常用的塑料;因此,降级PS成为一个关键的全局问题。在此,我们报道了假单胞菌铜绿假单胞菌菌株DSM 50071,最初从超虫,Zophobas Atratus的肠道分离,并通过Pseudomonass Sp降解Ps降解。 DSM 50071.我们使用电子显微镜检查PS劣化,并测量原子组合物的变化,以及PS表面上的水滴的接触角,表示从疏水性与亲水性的化学变化。我们通过X射线光电子能谱,傅立叶变换 - 红外光谱和核磁共振(NMR)进一步研究了化学结构变化,以在PS生物降解期间确认氧化途径中的羰基(C = O)的形成。在逆转录定量聚合酶链反应分析中,假单胞菌SP中丝氨酸水解酶(SH)的基因表达水平。 PS降解期间DSM 50071高度增加,SH抑制剂治疗试验进一步证实了PS的酶介导的生物降解。因此,这些发现的重要性超出了对Pseudomonas sp的新功能的发现。 DSM 50071在胆量的肠道中,突出了PS生物降解的潜在解决方案。

著录项

  • 来源
    《Environmental Science & Technology》 |2020年第11期|6987-6996|共10页
  • 作者单位

    School of Undergraduate Studies College of Transdisciplinary Studies Daegu Gyeongbuk Institute of Science and Technology Daegu 42988 Republic of Korea;

    School of Undergraduate Studies College of Transdisciplinary Studies Daegu Gyeongbuk Institute of Science and Technology Daegu 42988 Republic of Korea;

    School of Undergraduate Studies College of Transdisciplinary Studies Daegu Gyeongbuk Institute of Science and Technology Daegu 42988 Republic of Korea;

    School of Undergraduate Studies College of Transdisciplinary Studies Daegu Gyeongbuk Institute of Science and Technology Daegu 42988 Republic of Korea;

    School of Undergraduate Studies College of Transdisciplinary Studies and Department of Brain and Cognitive Sciences Graduate School Daegu Gyeongbuk Institute of Science and Technology Daegu 42988 Republic of Korea;

    Department of Chemistry Gwangju Institute of Science and Technology Gwangju 61005 Republic of Korea;

    School of Undergraduate Studies College of Transdisciplinary Studies Daegu Gveongbuk Institute of Science and Technology Daegu 42988 Republic of Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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