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首页> 外文期刊>Environmental Science & Technology >In Vitro Cytotoxicity and Risk Assessments of Environmental Pollutants Using Fibroblasts of a Stranded Finless Porpoise (Neophocaena asiaeorientalis)
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In Vitro Cytotoxicity and Risk Assessments of Environmental Pollutants Using Fibroblasts of a Stranded Finless Porpoise (Neophocaena asiaeorientalis)

机译：使用股线的剥离的剥离Porpoise的成纤维细胞（Neophocaena Asiaorientis）的环境污染物的体外细胞毒性和风险评估

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摘要

Cetaceans accumulate high levels of environmental pollutants, yet their toxicological studies have been difficult due to technical and ethical issues. It is essential to identify and fill the current knowledge gaps in the in vitro assays available for cetaceans. The present study establishes a novel in vitro assay that uses the fibroblasts of a finless porpoise (Neophocaena asiaeorientalis) (FF) stranded in the Seto Inland Sea (SIS) to answer questions about the cytotoxicity and risks of environmental pollutants. FF were treated with 17 compounds including polychlorinated biphenyls (PCBs) and dichlorodiphenyltrichloroethane and their metabolites (DDTs) and evaluated for cytotoxicity, viability, and apoptosis. The results of FF were compared with those of human fibroblasts (HF). The relative potencies of the test compounds were comparable between the two species, as EC_(50) of these compounds significantly correlated for FF and HF. Expo sure-activity ratios (EARs) revealed that accumulation of PCBs and DDTs are likely to pose adverse effects at the cellular level in the SIS finless porpoises, as their tissue concentrations exceeded EC_(50) values obtained in this study. This study successfully evaluated the risks of environmental pollutants using cetacean fibroblasts isolated by a non-invasive method that may be applied to various cetacean species and compounds.
机译：鲸尾草积累了高水平的环境污染物，但由于技术和道德问题，他们的毒理学研究一直很困难。必须识别和填补可用于鲸类的体外测定中的当前知识间隙。本研究建立了一种新的体外测定，它使用诸如濑户内海（SIS）中陷入困境的无翅流廊（Neophocaena Asiaeorientalis）（FF）的成纤维细胞来回答有关环境污染物的细胞毒性和风险的问题。用17种化合物处理FF，包括多氯联苯（PCB）和二氯二苯基三氯乙烷及其代谢物（DDTS），并评估细胞毒性，活力和凋亡。将FF的结果与人成纤维细胞（HF）进行比较。测试化合物的相对型效应与两种物种之间相当，因为这些化合物的EC_（50）对于FF和HF显着相关。 Expo确定 - 活性比（耳朵）显示PCB和DDT的积累可能在SIS F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F FONESSIONS造成不利影响，因为它们的组织浓度超过本研究中获得的EC_（50）值。该研究成功地评估了使用鲸类成纤维细胞的环境污染物的风险，其通过缺乏侵入性方法分离的鲸类成纤维细胞，其可用于各种唾液类物种和化合物。

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  • 来源
    《Environmental Science & Technology》 |2020年第11期|6832-6841|共10页
  • 作者

    Mari Ochiai; Nozomi Kurihara; Masashi Hirano; Akifumi Nakata; Hisato Iwata;

  • 作者单位

    Center for Marine Environmental Studies (CMES) Ehime University Matsuyama City Ehime 790-8577 Japan;

    Joint Faculty of Veterinary Medicine Yamaguchi University Yamaguchi City Yamaguchi 753-8515 Japan;

    Center for Marine Environmental Studies (CMES) Ehime University Matsuyama City Ehime 790-8577 Japan;

    Hokkaido University of Science Hokkaido 006-8585 Japan;

    Center for Marine Environmental Studies (CMES) Ehime University Matsuyama City Ehime 790-8577 Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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