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首页> 外文期刊>Environmental Science & Technology >Exposure to Environmental Levels of Pesticides Stimulates and Diversifies Evolution in Escherichia coli toward Higher Antibiotic Resistance
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Exposure to Environmental Levels of Pesticides Stimulates and Diversifies Evolution in Escherichia coli toward Higher Antibiotic Resistance

机译：暴露于环境水平的农药水平刺激和多样化大肠杆菌的进化升高抗生素抗性

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摘要

Antibiotic resistance is one of the most challenging issues in public health. Antibiotics have been increasingly used not only for humans and animals but also for crop protection as pesticides. Thus, antibiotics often coexist with pesticides in some environments. To investigate the effects of the co-occurring, nonantibiotic pesticides on the development of antibiotic resistance, we conducted long-term exposure experiments using an Escherichia coli K-12 model strain. The results reveal that (1) the exposure to pesticides (in mg/L) alone led to the emergence of mutants with significantly higher resistance to streptomycin; (2) the exposure to pesticides (in μg/L) together with a subinhibitory level (in high μg/L) of ampicillin synergistically stimulated the selection of ampicillin resistance and the cross-resistance to other antibiotics (i.e., ciprofloxacin, chloramphenicol, and tetracycline). Distinct and diversified genetic mutations emerged in the resistant mutants selected from the coexposure to both pesticides and ampicillin. The genetic mutations likely caused a holistic transcriptional regulation (e.g., biofilm formation, oxidative stress defense) when grown under antibiotic stress and led to increased antibiotic resistance. Together, these findings provide important fundamental insights into the development of antibiotic resistance and the resistance mechanisms under environmentally relevant conditions where antibiotics and nonantibiotic micropollutants coexist.
机译：抗生素抗性是公共卫生最具挑战性的问题之一。抗生素越来越多地用于人类和动物，也越来越多地用于作物保护作为农药。因此，抗生素通常在某些环境中与杀虫剂共存。为探讨共同发生的非抗生素农药对抗生素抗性发展的影响，我们使用大肠杆菌K-12模型菌株进行长期暴露实验。结果表明（1）单独暴露于农药（Mg / L）导致突变体的出现，具有明显更高的链霉素的抗性; （2）将农药（以μg/ L）暴露在氨苄青霉素的水平（高μg/ L）中，协同刺激氨苄青霉素抗性的选择和对其他抗生素的交叉抗性（即环氟苯甲酯，氯霉素和四环素）。在选自农药和氨苄青霉素的抗块中选择的抗性突变体中出现了不同的和多样化的遗传突变。当在抗生素应激下生长并导致增加抗生素抗性时，遗传突变可能导致整体转录调节（例如，生物膜形成，氧化胁迫防御）。这些发现在一起，为抗生素和非贪污微拷贝中共存的环境相关条件下的抗生素抗性和抗性机制提供了重要的根本洞察。

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  • 来源
    《Environmental Science & Technology》 |2020年第14期|8770-8778|共9页
  • 作者

    Yue Xing; Shuaiqi Wu; Yujie Men;

  • 作者单位

    Department of Civil and Environmental Engineering University of Illinois at Urbana-Champaign Urbana Illinois United States Department of Chemical and Environmental Engineering University of California Riverside California United States;

    Department of Civil and Environmental Engineering University of Illinois at Urbana-Champaign Urbana Illinois United States;

    Department of Chemical and Environmental Engineering University of California Riverside California United States Department of Civil and Environmental Engineering University of Illinois at Urbana-Champaign Urbana Illinois United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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