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首页> 外文期刊>Environmental Science & Technology >Mechanisms of pH-Dependent Uptake of lonizable Organic Chemicals by Fish from Oil Sands Process-Affected Water (OSPW)
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Mechanisms of pH-Dependent Uptake of lonizable Organic Chemicals by Fish from Oil Sands Process-Affected Water (OSPW)

机译：来自油砂过程影响的水（OSPW）对孤独有机化学品的pH依赖性摄取机制

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摘要

Uptake and effects of ionizable organic chemicals (IOCs) that are weak acids in aqueous solution by fish can differ as a function of pH. While the pH- dependent behavior of select IOCs is well-understood, complex mixtures of IOCs, e.g., from oil sands process-affected water (OSPW), have not yet been studied systematically. Here, we established an in vitro screening method using the rainbow trout gill cell line, RTgill-W1, to investigate pH-dependent cytotoxirity and permeation of IOCs across cultured epithelia using ultra-high-performance liquid chromatography with high-resolution mass spectrometry (UPLC-HRMS). The assay was benchmarked using model chemicals and technical mixtures, and then used to characterize fractions and reconstituted extracts of field-collected OSPW. Significant pH-dependent cytotoxicity of individual IOCs, acidic fractions, and reconstituted extracts of OSPW was observed. In vitro data were in good agreement with data from a 96 h in vivo exposure experiment with juvenile rainbow trout. Permeation of some IOCs from OSPW was mediated by active transport, as revealed by studies in which inhibitors of these active transport mechanisms were applied. We conclude that the RTgill-Wl in vitro assay is useful for the screening of pH-dependent uptake of IOCs in fish, and has applications for in vitro-in vivo extrapolation, and prioritization of chemicals in nontarget screenings.
机译：通过鱼水溶液中弱酸的可电离有机化学物质（IOC）的摄取和效果可以与pH的函数不同。虽然选择IOC的pH依赖性行为是良好的理解，但尚未系统地研究了IOC的复杂混合物，例如，来自油砂处理受影响的水（OSPW）。在这里，我们使用虹鳟鱼鳃细胞系RTGILL-W1建立了一种体外筛选方法，以研究使用超高效液相色谱法用高分辨率质谱法（UPLC）对培养的上皮细胞毒性和IOC渗透IOC的渗透性的筛选方法-hrms）。该测定采用模型化学品和技术混合物进行基准测试，然后用于表征级分和对现场收集的OSPW提取物。观察到各种IOC，酸性级分和OSPW的重构提取物的显着pH依赖性细胞毒性。体外数据与来自少年彩虹鳟鱼的体内曝光试验中的96小时数据吻合良好。来自OSPW的一些IOC的渗透是通过主动运输介导的，如应用这些活性运输机制的抑制剂的研究所揭示。我们得出结论，RTGILL-WL体外测定可用于筛选鱼类中的IOC的pH依赖性摄取，并具有体内体内外推的应用，以及非靶系中化学品的优先级。

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  • 来源
    《Environmental Science & Technology》 |2020年第15期|9547-9555|共9页
  • 作者

    Markus Brinkmann; Hattan Alharbi; Ulyana Fuchylo; Steve Wiseman; Garrett Morandi; Hui Peng; John P. Giesy; Paul D. Jones; Markus Hecker;

  • 作者单位

    School of Environment and Sustainability (SENS) Toxicology Centre and Global Institutes for Water Security (GIWS) University of Saskatchewan Saskatoon S7N SC8 Canada;

    Department of Plant Protection College of Food and Agriculture Sciences King Saud University Riyadh 11451 Saudi Arabia;

    Toxicology Centre University of Saskatchewan Saskatoon S7N 5B3 Canada;

    Department of Biological Sciences University of Lethbridge Lethbridge T1K 3M4 Canada;

    Toxicology Centre University of Saskatchewan Saskatoon S7N 5B3 Canada;

    Toxicology Centre University of Saskatchewan Saskatoon S7N 5B3 Canada Department of Chemistry University of Toronto Toronto M5S 3H6 Canada;

    Toxicology Centre and Department of Veterinary Biomedkal Sciences University of Saskatchewan Saskatoon S7N 5B3 Canada Department of Zoology and Center for Integrative Toxicology Michigan State University East Lansing Michigan 48824 United States Department of Environmental Sciences Baylor University Waco Texas 76706 United States;

    School of Environment and Sustainability (SENS) and Toxicology Centre University of Saskatchewan Saskatoon S7N 5C8 Canada;

    School of Environment and Sustainability (SENS) and Toxicology Centre University of Saskatchewan Saskatoon S7N 5C8 Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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