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首页> 外文期刊>Environmental Science & Technology >Compromised Autophagic Effect of Polystyrene Nanoplastics Mediated by Protein Corona Was Recovered after Lysosomal Degradation of Corona
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Compromised Autophagic Effect of Polystyrene Nanoplastics Mediated by Protein Corona Was Recovered after Lysosomal Degradation of Corona

机译：在溶酶体降解电晕的溶酶体降解后，回收了蛋白质电晕介导的聚苯乙烯纳米本体的自噬作用受损

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摘要

The adverse biological and ecological consequences of plastic debris have become a serious problem worldwide. Evidences have uncovered the accumulation of nanoplastics (NPs) in organisms. In a complex biological environment, proteins are prone to adsorbed onto the NPs' surface and form a protein corona layer, which mediates the interaction of NPs with cells. Here, we discovered the interaction of polystyrene (PS) NPs with protein fetal bovine serum (FBS) and altered cytotoxic effects. Mechanistically, prefabricated FBS protein corona mediated the relief of autophagic flux blockage, autophagosomes accumulation, and lysosomal damage in RAW264.7 cells caused by PS NPs. Using an individual fluorescent protein bovine serum albumin (BSA) as a corona surrogate, we demonstrated that coronal BSA remains, at least partially, on the surface of PS NPs during the initial stage of internalization and protects cell membrane from PS NPs-induced damage. However, along with the degradation of corona in lysosomes, reappearance of cytotoxicity was observed. Herein, we provided a proof of principle of the manipulation of corona on NPs' toxicity and we expect the result will promote the further safety assessment of NPs.
机译：塑料碎片的不利生物和生态后果已成为全球的严重问题。证据已经揭示了纳米型体（NPS）在生物体中的积累。在复杂的生物环境中，蛋白质易于吸附到NPS的表面上并形成蛋白质电晕层，其介导NP与细胞的相互作用。这里，我们发现聚苯乙烯（PS）NP与蛋白质胎儿牛血清（FBS）的相互作用，并改变细胞毒性作用。机械上，预制的FBS蛋白质电晕介导的自噬助焊剂堵塞，自噬囊体积聚和PS NPS引起的Raw264.7细胞中的溶酶体损伤的缓解。使用单独的荧光蛋白牛血清白蛋白（BSA）作为电晕替代物，我们证明冠状BSA至少部分地在内化的初始阶段期间至少部分地在PS NPS的表面上，并保护来自PS NPS诱导的损伤的细胞膜。然而，随着溶酶体中的冠状动脉的降解，观察到细胞毒性的重新出现。在此，我们提供了对NPS毒性进行了操纵原则的证据，我们预计结果将促进NPS的进一步安全评估。

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  • 来源
    《Environmental Science & Technology》 |2020年第18期|11485-11493|共9页
  • 作者

    Ya Tan; Xiangyu Zhu; Di Wu; Erqun Song; Yang Song;

  • 作者单位

    Key Laboratory of Luminescence Analysis and Molecular Sensing Ministry of Education College of Pharmaceutical Sciences Southwest University Chongqing 40071S People's Republic of China;

    Key Laboratory of Luminescence Analysis and Molecular Sensing Ministry of Education College of Pharmaceutical Sciences Southwest University Chongqing 4007J5 People's Republic of China;

    Key Laboratory of Luminescence Analysis and Molecular Sensing Ministry of Education College of Pharmaceutical Sciences Southwest University Chongqing 400715 People's Republic of China;

    Key Laboratoiy of Luminescence Analysis and Molecular Sensing Ministry of Education College of Pharmaceutical Sciences Southwest University Chongqing 400715 People's Republic of China;

    Key Laboratory of Luminescence Analysis and Molecular Sensing Ministry of Education College of Pharmaceutical Sciences Southwest University Chongqing 400715 People's Republic of China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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