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首页> 外文期刊>Environmental Science & Technology >Variability in Arsenic Methylation Efficiency across Aerobic and Anaerobic Microorganisms
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Variability in Arsenic Methylation Efficiency across Aerobic and Anaerobic Microorganisms

机译：有氧和厌氧微生物砷甲基化效率的可变性

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摘要

Microbially-mediated methylation of arsenic (As) plays an important role in the As biogeochemical cycle, particularly in rice paddy soils where methylated As, generated microbially, is translocated into rice grains. The presence of the arsenite (As(Ⅲ)) methyltransferase gene (arsM) in soil microbes has been used as an indication of their capacity for As methylation. Here, we evaluate the ability of seven microorganisms encoding active ArsM enzymes to methylate As. Amongst those, only the aerobic species were efficient methylators. The anaerobic microorganisms presented high resistance to As exposure, presumably through their efficient As(Ⅲ) efflux, but methylated As poorly. The only exception were methanogens, for which efficient As methylation was seemingly an artifact of membrane disruption. Deletion of an efflux pump gene (acr3) in one of the anaerobes, Clostridium pasteurianum, rendered the strain sensitive to As and capable of more efficiently methylating As. Our results led to the following conclusions: (ⅰ) encoding a functional ArsM enzyme does not guarantee that a microorganism will actively drive As methylation in the presence of the metalloid and (ⅱ) there is an inverse relationship between efficient microbial As efflux and its methylation, because the former prevents the intracellular accumulation of As.
机译：微生物介导的砷（AS）在作为生物地理细胞循环中起重要作用，特别是在稻米稻草中，其中甲基化为微生物产生的，易转化为水稻颗粒。在土壤微生物中存在砷酸盐（AS（Ⅲ））甲基转移酶基因（ARSM）被用作它们作为甲基化的能力的指示。在这里，我们评估将七种微生物编码为甲酸甲酯的七种微生物的能力。在那些中，只有有氧物种是有效的甲基。厌氧微生物呈现出高抗性的抗曝光，大概是通过其有效的（Ⅲ）流出，但甲基化为差。唯一的例外是甲烷，因为甲基化的效率是似乎是膜破坏的伪影。缺失在一个厌氧物，梭菌裤氏菌中的流出泵基因（ACR3），使应变为敏感，并能够更有效地甲基化。我们的结果导致了以下结论：（Ⅰ）编码功能性ARSM酶并不能保证在金属体存在下，微生物在甲基化中被激活地驱动，并且（Ⅱ）在有效的微生物和其甲基化之间存在反比关系。 ，因为前者防止了细胞内积聚的。

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  • 来源
    《Environmental Science & Technology》 |2020年第22期|14343-14351|共9页
  • 作者

    Karen Viacava; Karin Lederballe Meibom; David Ortega; Shannon Dyer; Arnaud Gelb; Leia Falquet; Nigel P. Minton; Adrien Mestrot; Rizlan Bernier-Latmani;

  • 作者单位

    Environmental Microbiology Laboratory School of Architecture Civil and Environmental Engineering Ecole Polytechnique Federate de Lausanne 1015 Lausanne Switzerland;

    Environmental Microbiology Laboratory School of Architecture Civil and Environmental Engineering Ecole Polytechnique Federate de Lausanne 1015 Lausanne Switzerland;

    Clostridia Research Group BBSRC/EPSRC Synthetic Biology Research Centre (SBRC) School of Life Sciences Centre for Biomolecular Sciences University of Nottingham NG7 2RD Nottingham United Kingdom;

    Environmental Microbiology Laboratory School of Architecture Civil and Environmental Engineering Ecole Polytechnique Federate de Lausanne 1015 Lausanne Switzerland;

    Laboratory for Environmental Biotechnology School of Architecture Civil and Environmental Engineering Ecole Polytechnique Federate de Lausanne 1015 Lausanne Switzerland;

    Environmental Microbiology Laboratory School of Architecture Civil and Environmental Engineering Ecole Polytechnique Federate de Lausanne 1015 Lausanne Switzerland;

    Clostridia Research Group BBSRC/EPSRC Synthetic Biology Research Centre (SBRC) School of Life Sciences Centre for Biomolecular Sciences University of Nottingham NG7 2RD Nottingham United Kingdom;

    Institute of Geography University of Bern 3012 Bern Switzerland;

    Environmental Microbiology Laboratory School of Architecture Civil and Environmental Engineering Ecole Polytechnique Federate de Lausanne 1015 Lausanne Switzerland;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Arsenic methylation; arsenic detoxification; arsenite efflux; Acr3 enzyme; microbial warfare; arsenical antibiotics;

    机译：砷甲基化;砷解毒;阿森斯·雷化;ACR3酶;微生物战;砷抗生素;

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