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首页> 外文期刊>Environmental Science & Technology >Wavelength-Dependent Damage to Adenoviral Proteins Across the Germicidal UV Spectrum
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Wavelength-Dependent Damage to Adenoviral Proteins Across the Germicidal UV Spectrum

机译：整个杀菌紫外光谱中腺病毒蛋白的波长依赖性损伤

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src="http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/esthag/2018/esthag.2018.52.issue-1/acs.est.7b04602/20171226/images/medium/es-2017-04602x_0007.gif">Adenovirus, a waterborne pathogen responsible for causing bronchitis, pneumonia, and gastrointestinal infections, is highly resistant to UV disinfection and therefore drives the virus disinfection regulations set by the U.S. Environmental Protection Agency. Polychromatic UV irradiation has been shown to be more effective at inactivating adenovirus and other viruses than traditional monochromatic irradiation emitted at 254 nm; the enhanced efficacy has been attributed to UV-induced damage to viral proteins. This research shows UV-induced damage to adenoviral proteins across the germicidal UV spectrum at wavelength intervals between 200 and 300 nm. A deuterium lamp with bandpass filters and UV light-emitting diodes (UV LEDs) isolated wavelengths in approximate 10 nm intervals. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and image densitometry were used to detect signatures for the hexon, penton, fiber, minor capsid, and core proteins. The greatest loss of protein signature, indicating damage to viral proteins, occurred below 240 nm. Hexon and penton proteins exposed to a dose of 28 mJ/cm2 emitted at 214 nm were approximately 4 times as sensitive and fiber proteins approximately 3 times as sensitive as those exposed to a dose of 50 mJ/cm2 emitted at 254 nm. At 220 nm, a dose of 38 mJ/cm2 reduced the hexon and penton protein quantities to approximately 33% and 31% of the original amounts, respectively. In contrast, a much higher dose of 400 mJ/cm2 emitted at 261 and 278 nm reduced the original protein quantity to between 66–89% and 80–93%, respectively. No significant damage was seen with a dose of 400 mJ/cm2 at 254 nm. This research directly correlates enhanced inactivation at low wavelengths with adenoviral protein damage at those wavelengths, adding fundamental insight into the mechanisms of inactivation of polychromatic germicidal UV irradiation for improving UV water disinfection.
机译：src =“ http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/esthag/2018/esthag.2018.52.issue-1/acs.est.7b04602/20171226/images/medium /es-2017-04602x_0007.gif“>腺病毒是一种引起支气管炎，肺炎和胃肠道感染的水生病原体，对紫外线消毒具有高度抵抗力，因此推动了美国环境保护署制定的病毒消毒法规。已证明，多色UV辐射比254 nm处发射的传统单色辐射更能灭活腺病毒和其他病毒。功效增强归因于紫外线引起的病毒蛋白损伤。这项研究表明，紫外线在200至300 nm的波长间隔内对整个杀菌UV光谱的腺病毒蛋白造成损害。具有带通滤光片和紫外线发光二极管（UV LED）的氘灯以大约10 nm的间隔隔离波长。十二烷基硫酸钠聚丙烯酰胺凝胶电泳和图像光密度法用于检测六邻体，戊烯，纤维，次要衣壳和核心蛋白的特征。蛋白质标记的最大损失表明对病毒蛋白质的损害，发生在240 nm以下。暴露于214 nm的28 mJ / cm 2 剂量的六邻体和戊烯蛋白的敏感度是暴露于50 mJ / cm的六倍体蛋白和纤维蛋白的3倍 2 在254 nm处发射。在220 nm处，剂量为38 mJ / cm 2 将六邻体和五通体蛋白的量分别减少到原始量的33％和31％。相比之下，在261和278 nm处发射的更高剂量的400 mJ / cm 2 将原始蛋白质量分别降低到66-89％和80-93％之间。在254 nm处，剂量为400 mJ / cm 2 ，未见明显损伤。这项研究将低波长下的增强灭活与这些波长上的腺病毒蛋白损伤直接相关，从而为多色杀菌紫外线照射的灭活机理提供了基础性见解，以改善紫外线水的消毒效果。

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  • 来源
    《Environmental Science & Technology》 |2018年第1期|223-229|共7页
  • 作者

    Sara E. Beck; Natalie M. Hull; Christopher Poepping; Karl G. Linden;

  • 作者单位

    Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, Colorado 80309, United States;

    Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, Colorado 80309, United States;

    Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, Colorado 80309, United States;

    Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, Colorado 80309, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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