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首页> 外文期刊>Environmental Science & Technology >Synthetic Phenolic Antioxidants Cause Perturbation in Steroidoqenesis in Vitro and in Vivo
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Synthetic Phenolic Antioxidants Cause Perturbation in Steroidoqenesis in Vitro and in Vivo

机译：合成酚类抗氧化剂在体内和体外引起类固醇的摄动

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src="http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/esthag/2018/esthag.2018.52.issue-2/acs.est.7b05057/20180111/images/medium/es-2017-05057r_0007.gif">Synthetic phenolic antioxidants (SPAs) are closely correlated with human life due to their extensive usages, and increasing concerns have been raised on their biosafety. The previous controversial findings caused continuous debates on their potential endocrine disrupting effects. In the present study, four commonly used SPAs, including butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), tert-butyl hydroquinone (TBHQ) and 2,2′-methylenebis(6-tert-butyl-4-methylphenol) (AO2246), were investigated for their estrogenic effects, and the results from in vitro screening assays showed SPAs themselves had negligible estrogen receptor binding affinities. Nevertheless, significant increase in E2 secretion was observed in H295R cells treated with SPAs, especially for BHA. The transcriptional levels of steroidogenic enzymes, including StAR, 3βHSD, CYP11B1, and CYP11B2 were up-regulated via the mediation of protein kinase A (PKA) signaling pathway. In vivo experiment confirmed that waterborne exposure to BHA disturbed E2 and testosterone (T) levels in zebrafish gonad, thus causing potential estrogenic effects through the regulation of hypothalamic-pituitary–gonadal-liver axis (HPGL-axis). Accordingly, this study has provided new insights for SPA-induced endocrine disrupting effects. Considering the allowable maximum level of individual BHA or in combination with TBHQ and BHT in foodstuffs (200 mg kg–1), the perturbation in steroidogenesis observed for relatively low concentrations of SPAs would need more public attention.
机译：src =“ http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/esthag/2018/esthag.2018.52.issue-2/acs.est.7b05057/20180111/images/medium /es-2017-05057r_0007.gif“>合成酚类抗氧化剂（SPA）的广泛使用与人类生活息息相关，人们对其生物安全性的关注也越来越高。先前有争议的发现引起了关于其潜在内分泌干扰作用的持续争论。在本研究中，四种常用的SPA包括丁基化羟基茴香醚（BHA），丁基化羟基甲苯（BHT），叔丁基对苯二酚（TBHQ）和2,2'-亚甲基双（6- 对叔丁基-4-甲基苯酚（AO2246）的雌激素作用进行了研究，体外筛选试验结果表明，SPAs自身的雌激素受体结合亲和力可忽略不计。然而，在SPAs处理的H295R细胞中，尤其是对BHA，观察到E 2 分泌的显着增加。通过蛋白激酶A（PKA）信号通路的介导，类固醇生成酶（包括StAR，3βHSD，CYP11B1和CYP11B2）的转录水平上调。体内实验证实，水暴露于BHA会干扰斑马鱼性腺中的E 2 和睾丸激素（T）水平，从而通过调节下丘脑-垂体-性腺-肝轴（HPGL-轴）引起潜在的雌激素作用）。因此，该研究为SPA诱导的内分泌干扰作用提供了新的见解。考虑到食品中单个BHA的最高允许水平或与TBHQ和BHT结合使用（200 mg kg –1 ），相对较低的SPA浓度所观察到的类固醇生成扰动将需要引起更多公众关注。

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  • 来源
    《Environmental Science & Technology》 |2018年第2期|850-858|共9页
  • 作者

    Xiaoxi Yang; Wenting Song; Na Liu; Zhendong Sun; Ruirui Liu; Qian S. Liu; Qunfang Zhou; Guibin Jiang;

  • 作者单位

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,Medical College, Henan Polytechnic University, Jiaozuo 454000, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,School of Life Science, Shanxi University, Taiyuan 030006, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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