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首页> 外文期刊>Environmental Science & Technology >Detection of Cyanobacteria in Eutrophic Water Using a Portable Electrocoagulator and NanoGene Assay
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Detection of Cyanobacteria in Eutrophic Water Using a Portable Electrocoagulator and NanoGene Assay

机译：便携式电凝仪和纳米基因检测法检测富营养化水中的蓝细菌

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摘要

We have demonstrated the detection of cyanobacteria in eutrophic water samples using a portable electrocoagulator and NanoGene assay. The electrocoagulator is designed to preconcentrate cyanobacteria from water samples prior to analysis via NanoGene assay. Using Microcystis aeruginosa laboratory culture and environmental samples (cell densities ranging from 1.7 × 10~(5) to 4.1 × 10~(6) and 6.5 × 10~(3) to 6.6 × 10~(7) cells·mL~(–1), respectively), the electrocoagulator was evaluated and compared with a conventional centrifuge. Varying the operation duration from 0 to 300 s with different cell densities was first investigated. Preconcentration efficiencies (obtained via absorbance measurement) and dry cell weight of preconcentrated cyanobacteria were then obtained and compared. For laboratory samples at cell densities from 3.2 × 10~(5) to 4.1 × 10~(6) cells·mL~(–1), the preconcentration efficiencies of electrocoagulator appeared to be stable at ∼60%. At lower cell densities (1.7 and 2.2 × 10~(5) cells·mL~(–1)), the preconcentration efficiencies decreased to 33.9 ± 0.2 and 40.4 ± 5.4%, respectively. For environmental samples at cell densities of 2.7 × 10~(5) and 6.6 × 10~(7) cells·mL~(–1), the electrocoagulator maintained its preconcentration efficiency at ∼60%. On the other hand, the centrifuge’s preconcentration efficiencies decreased to nondetectable and below 40%, respectively. This shows that the electrocoagulator outperformed the centrifuge when using eutrophic water samples. Finally, the compatibility of the electrocoagulator with the NanoGene assay was verified via the successful detection of the microcystin synthetase D ( mcyD ) gene in environmental samples. The viability of the electrocoagulator as an in situ compatible alternative to the centrifuge is also discussed.
机译：我们已经证明了使用便携式电凝器和NanoGene检测技术可以检测富营养化水样中的蓝细菌。电凝器设计用于在通过NanoGene分析进行分析之前从水样本中预浓缩蓝细菌。使用铜绿微囊藻实验室培养和环境样品（细胞密度范围为1.7×10〜（5）至4.1×10〜（6）和6.5×10〜（3）至6.6×10〜（7）细胞·mL〜（– 1）），评估电凝器并与常规离心机进行比较。首先研究了从0到300 s在不同细胞密度下的持续时间。然后获得并比较预浓缩效率（通过吸光度测量获得）和预浓缩蓝细菌的干细胞重量。对于细胞密度从3.2×10〜（5）到4.1×10〜（6）个细胞·mL〜（-1）的实验室样品，电凝器的预浓缩效率似乎稳定在〜60％。在较低的细胞密度（1.7和2.2×10〜（5）个细胞·mL〜（–1））下，预浓缩效率分别降至33.9±0.2和40.4±5.4％。对于细胞密度为2.7×10〜（5）和6.6×10〜（7）个细胞·mL〜（-1）的环境样品，电凝器的预浓缩效率保持在约60％。另一方面，离心机的预浓缩效率分别降至无法检测和低于40％。这表明使用富营养水样品时电凝器的性能优于离心机。最后，通过成功检测环境样品中的微囊藻毒素合成酶D（mcyD）基因，验证了电凝剂与NanoGene检测的相容性。还讨论了电凝器作为离心机的现场兼容替代品的可行性。

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  • 来源
    《Environmental Science & Technology》 |2018年第3期|1375-1385|共11页
  • 作者

    Eun-Hee Lee; Beelee Chua; Ahjeong Son;

  • 作者单位

    Department of Environmental Science and Engineering, Ewha Womans University, Seoul, Republic of Korea;

    School of Electrical Engineering, Korea University, Seoul, Republic of Korea;

    Department of Environmental Science and Engineering, Ewha Womans University, Seoul, Republic of Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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