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首页> 外文期刊>Environmental Science & Technology >Adenovirus Replication Cycle Disruption from Exposure to Polychromatic Ultraviolet Irradiation
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Adenovirus Replication Cycle Disruption from Exposure to Polychromatic Ultraviolet Irradiation

机译：从暴露到多色紫外线辐照的腺病毒复制周期破坏。

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摘要

Polychromatic ultraviolet (UV) light with bandwidth of 20 nm and peak emission centered at 224, 254, or 280 nm (UV_(224), UV_(254), and UV_(280), respectively) were used to inactivate human adenovirus type 2 (HAdV-2). Quantitative polymerase chain reaction (qPCR) and reverse transcriptase qPCR assays were used to elucidate the step in the HAdV-2 replication cycle that was disrupted after UV exposure. UV treatment at any of the wavelengths analyzed did not inhibit association of HAdV-2 to the host cells even after exposure to a fluence (UV dose) that would produce a virus inactivation efficiency, measured by plaque assay, greater than 99.99%. In contrast, UV irradiation at all three peak emissions disrupted early E1A gene transcription and viral DNA replication, but different mechanisms appeared to be dominating such disruptions. UV_(224) seemed to have little effect on the integrity of the viral genome but produced a structural transformation of the viral capsid that may inhibit the delivery of viral genome into the host cell nucleus. On the other hand, UV_(254) and UV_(280) did not affect the integrity of the viral capsid, but the mutations they produced on the viral genome might cause the inhibition of the early gene transcription and DNA replication after the viral genome successfully translocated into the host cell nucleus.
机译：使用带宽为20 nm且峰值发射集中在224、254或280 nm（分别为UV_（224），UV_（254）和UV_（280））的多色紫外（UV）光灭活2型人类腺病毒。 （HAdV-2）。定量聚合酶链反应（qPCR）和逆转录酶qPCR分析用于阐明HAdV-2复制周期中受紫外线照射破坏的步骤。即使在暴露于通量（UV剂量）下，通过斑块测定法测得的通量（UV剂量）大于99.99％，使用任何分析波长的UV处理也不会抑制HAdV-2与宿主细胞的缔合。相反，在所有三个峰值发射处的紫外线照射都破坏了早期E1A基因的转录和病毒DNA的复制，但不同的机制似乎主导了这种破坏。 UV_（224）似乎对病毒基因组的完整性几乎没有影响，但是产生了病毒衣壳的结构转化，这可能抑制了病毒基因组向宿主细胞核内的传递。另一方面，UV_（254）和UV_（280）不会影响病毒衣壳的完整性，但是它们在病毒基因组上产生的突变可能会抑制病毒基因组成功后的早期基因转录和DNA复制。易位到宿主细胞核中。

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  • 来源
    《Environmental Science & Technology》 |2018年第6期|3652-3659|共8页
  • 作者

    Bernardo Vazquez-Bravo; Kelley Gonçalves; Joanna L. Shisler; Benito J. Mariñas;

  • 作者单位

    Department of Civil and Environmental Engineering, Department of Microbiology and College of Medicine, and Safe Global Water Institute, University of Illinois at Urbana—Champaign, Urbana, Illinois 61801, United States;

    Department of Civil and Environmental Engineering, Department of Microbiology and College of Medicine, and Safe Global Water Institute, University of Illinois at Urbana—Champaign, Urbana, Illinois 61801, United States;

    Department of Civil and Environmental Engineering, Department of Microbiology and College of Medicine, and Safe Global Water Institute, University of Illinois at Urbana—Champaign, Urbana, Illinois 61801, United States;

    Department of Civil and Environmental Engineering, Department of Microbiology and College of Medicine, and Safe Global Water Institute, University of Illinois at Urbana—Champaign, Urbana, Illinois 61801, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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