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首页> 外文期刊>Environmental Science & Technology >Does Size Matter? An Experimental Evaluation of the Relative Abundance and Decay Rates of Aquatic Environmental DNA
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Does Size Matter? An Experimental Evaluation of the Relative Abundance and Decay Rates of Aquatic Environmental DNA

机译：尺寸重要吗？水生环境DNA相对丰度和衰减率的实验评估

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摘要

Environmental DNA (eDNA) is increasingly used to monitor aquatic macrofauna. Typically, short mitochondrial DNA fragments are targeted because these should be relatively more abundant in the environment as longer fragments will break into smaller fragments over time. However, longer fragments may permit more flexible primer design and increase taxonomic resolution for eDNA metabarcoding analyses, and recent studies have shown that long mitochondrial eDNA fragments can be extracted from environmental water samples. Nuclear eDNA fragments have also been proposed as targets, but little is known about their persistence in the aquatic environment. Here we measure the abundance of mitochondrial eDNA fragments of different lengths and of short nuclear eDNA fragments, originating from captive fish in experimental tanks, and we test whether longer mitochondrial and short nuclear fragments decay faster than short mitochondrial fragments following fish removal. We show that when fish are present, shorter mitochondrial fragments are more abundant in water samples than both longer mitochondrial fragments and short nuclear eDNA fragments. However, the rate of decay following fish removal was similar for all fragment types, suggesting that the differences in abundance resulted from differences in the rates at which different fragment types were produced rather than differences in their decay rates.
机译：环境DNA（eDNA）越来越多地用于监测水生大型动物。通常，针对短线粒体DNA片段是有针对性的，因为随着时间的推移，较长的片段会分解为较小的片段，因此它们在环境中应相对丰富。但是，更长的片段可能允许更灵活的引物设计，并提高eDNA元条形码分析的分类学分辨率，最近的研究表明，可以从环境水样中提取长线粒体eDNA片段。还提出了核eDNA片段作为靶标，但对其在水生环境中的持久性知之甚少。在这里，我们测量了不同长度的线粒体eDNA片段和短核eDNA片段的丰度，这些片段源自实验鱼缸中的圈养鱼，并且测试了去除鱼后较长的线粒体和短核片段的衰变是否比短线粒体片段快。我们表明，当鱼类存在时，较短的线粒体片段比较长的线粒体片段和较短的核eDNA片段都富集。但是，对于所有碎片类型，去除鱼后的腐烂速率都相似，这表明丰度差异是由产生不同碎片类型的速率差异而不是其腐烂速率差异引起的。

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  • 来源
    《Environmental Science & Technology》 |2018年第11期|6408-6416|共9页
  • 作者

    Jonas Bylemans; Elise M. Furlan; Dianne M. Gleeson; Christopher M. Hardy; Richard P. Duncan;

  • 作者单位

    Institute for Applied Ecology and Invasive Animals Cooperative Research Centre, University of Canberra, Canberra, Australian Capital Territory 2617, Australia;

    Institute for Applied Ecology and Invasive Animals Cooperative Research Centre, University of Canberra, Canberra, Australian Capital Territory 2617, Australia;

    Institute for Applied Ecology and Invasive Animals Cooperative Research Centre, University of Canberra, Canberra, Australian Capital Territory 2617, Australia;

    Institute for Applied Ecology and Invasive Animals Cooperative Research Centre, University of Canberra, Canberra, Australian Capital Territory 2617, Australia,Commonwealth Scientific and Industrial Research Organisation Land and Water, General Post Office Box 1700, Canberra, Australian Capital Territory 2601, Australia;

    Institute for Applied Ecology and Invasive Animals Cooperative Research Centre, University of Canberra, Canberra, Australian Capital Territory 2617, Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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