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首页> 外文期刊>Environmental Science & Technology >Bioavailability of Pyrene Associated with Different Types of Protein Compounds: Direct Evidence for Its Uptake by Daphnia magna
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Bioavailability of Pyrene Associated with Different Types of Protein Compounds: Direct Evidence for Its Uptake by Daphnia magna

机译：与不同类型的蛋白质化合物相关的P的生物利用度：大型蚤（Daphnia magna）吸收的直接证据。

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摘要

The protein-like dissolved organic matter (DOM) is ubiquitous in aquatic environments. However, the bioavailability of protein-like DOM-associated hydrophobic organic compounds (HOCs) is not well-understood, and in particular, the direct evidence of their uptake by organisms is scarce. In the present work, tryptone (2000 Da), bovine serum albumin (BSA; 66 000 Da), and phycocyanin (120 000 Da) were chosen as model protein-like DOM, which were labeled by commercial fluorescein (cy5) to investigate the uptake mechanisms of protein compound-associated pyrene (a typical HOC) by Daphnia magna. The pyrene concentration in the tissues except the gut and immobilization of D. magna were detected to calculate the bioavailable fraction of protein compound-associated pyrene when the freely dissolved pyrene concentration was controlled through passive dosing devices. The results demonstrated that the tryptone could permeate cellular membrane and directly enter the tissues of D. magna from the exposure solutions, whereas BSA and phycocyanin might indirectly enter the tissues from the gut. A part of pyrene associated with protein compounds was bioavailable to D. magna; the order of their bioavailable fractions was trypone (54.6-58.1%) phycocyanin (21.6-32.8%) BSA (17.7-26.8%). The difference was principally related to the uptake mechanisms of pyrene associated with different types of protein. This work suggests that the protein compound-associated HOCs should be considered to evaluate the bioavailability and eco-environmental hazard of HOCs in natural waters.
机译：蛋白质样溶解的有机物（DOM）在水生环境中无处不在。但是，蛋白质类DOM相关的疏水性有机化合物（HOCs）的生物利用度尚未得到很好的理解，特别是缺乏有机体对其吸收的直接证据。在目前的工作中，选择胰蛋白tone（2000 Da），牛血清白蛋白（BSA; 66 000 Da）和藻蓝蛋白（120 000 Da）作为模型蛋白样DOM，并用商品荧光素（cy5）标记以研究大型蚤（Daphnia magna）对蛋白质化合物相关的asso（典型的HOC）的吸收机制。当通过被动加料装置控制自由溶解的concentration浓度时，检测肠道和and的固定化except以外的组织中pyr的浓度，以计算与蛋白质化合物相关的pyr的生物利用度。结果表明，类胰蛋白could可以透过暴露液渗透到细胞膜中并直接进入大果蝇的组织，而牛血清白蛋白和藻蓝蛋白则可能从肠道间接进入组织。与蛋白质化合物相关的部分of可被D. magna生物利用。它们的生物利用级分的顺序是胰蛋白p（54.6-58.1％）>藻蓝蛋白（21.6-32.8％）> BSA（17.7-26.8％）。差异主要与与不同类型蛋白质相关的of的吸收机制有关。这项工作表明应考虑与蛋白质化合物相关的HOC，以评估天然水中HOC的生物利用度和生态环境危害。

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  • 来源
    《Environmental Science & Technology》 |2018年第17期|9851-9860|共10页
  • 作者

    Lin Hui; Xia Xinghui; Jiang Xiaoman; Bi Siqi; Wang Haotian; Zhai Yawei; Wen Wu; Guo Xuejun;

  • 作者单位

    Beijing Normal Univ, Sch Environm, State Key Lab Water Environm Simulat, Beijing 100875, Peoples R China;

    Beijing Normal Univ, Sch Environm, State Key Lab Water Environm Simulat, Beijing 100875, Peoples R China;

    Beijing Normal Univ, Sch Environm, State Key Lab Water Environm Simulat, Beijing 100875, Peoples R China;

    Beijing Normal Univ, Sch Environm, State Key Lab Water Environm Simulat, Beijing 100875, Peoples R China;

    Beijing Normal Univ, Sch Environm, State Key Lab Water Environm Simulat, Beijing 100875, Peoples R China;

    Beijing Normal Univ, Sch Environm, State Key Lab Water Environm Simulat, Beijing 100875, Peoples R China;

    Beijing Normal Univ, Sch Environm, State Key Lab Water Environm Simulat, Beijing 100875, Peoples R China;

    Beijing Normal Univ, Sch Environm, State Key Lab Water Environm Simulat, Beijing 100875, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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