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首页> 外文期刊>Environmental Science & Technology >A Rieske-Type Oxygenase of Pseudomonas sp. BIOMIG1 Converts Benzalkonium Chlorides to Benzyldimethyl Amine
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A Rieske-Type Oxygenase of Pseudomonas sp. BIOMIG1 Converts Benzalkonium Chlorides to Benzyldimethyl Amine

机译：假单胞菌属的Rieske型加氧酶。 BIOMIG1将苯扎氯铵转化为苄基二甲基胺

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摘要

Recently, an array of eight genes involved in the biotransformation of benzalkonium chlorides (BACs)-an active ingredient of many disinfectants-to benzyldimethyl amine (BDMA) was identified in the genome of Pseudomonas sp. BIOMIG1, which is a bacterium present in various environments and mineralizes BACs. In this study, we showed that heterologous egression of an oxygenase gene (oxyBAC) present in this gene array in E. coli resulted in formation of BDMA from BACs at a rate of 14 µM h~(-1). oxyBAC is phylogenetically classified as a Rieske-type oxygenase (RO) and belongs to a group which catalyzes the cleavage of C-N~+ bond between either methl or alkyl ester and a quaternary nitrogen (N) of natural quaternary ammonium compounds such as stadiydrine, carnitine, and trimethylglycine. Insertion of two glycines into the Rieske domain and substitution of tyrosine with leucine in the mononudear iron center differentiate oxyBAC from other ROs that cleave C-N~+, and presumably facilitate the cleavage of saturated alkyl chain from quaternary N via N-deaUylation reaction. In addition, unlike other ROs, oxyBAC did not require a specific reductase to function. Our results demonstrate that oxyBAC represents a new member of RO associated with BAC degradation, and have applications for controlling the fate of BACs in the environment
机译：最近，在假单胞菌属（Pseudomonas sp。）的基因组中鉴定了涉及八个苯扎氯铵（BAC）-许多消毒剂的活性成分-生物转化为苄基二甲基胺（BDMA）的八个基因。 BIOMIG1是存在于各种环境中的细菌，可使BAC矿化。在这项研究中，我们表明存在于大肠杆菌中此基因阵列中的加氧酶基因（oxyBAC）的异源释放导致以14 µM h〜（-1）的速率从BAC形成BDMA。 oxyBAC在系统发生上被分类为Rieske型加氧酶（RO），并且属于一个基团，该基团催化methl或烷基酯与天然季铵化合物（如斯坦地丁，肉碱）的季氮（N）之间的CN〜+键裂解和三甲基甘氨酸。在单核铁中心将两个甘氨酸插入Rieske结构域并用亮氨酸取代酪氨酸，将oxyBAC与其他裂解C-N〜+的RO区别开来，大概是通过N-脱酰化反应促进了饱和烷基链从季N上的裂解。此外，与其他RO不同，oxyBAC不需要特定的还原酶即可发挥作用。我们的结果表明，oxyBAC代表与BAC降解相关的RO的新成员，并在控制环境中BAC的命运方面具有应用

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  • 来源
    《Environmental Science & Technology》 |2017年第1期|175-181|共7页
  • 作者

    Emine Ertekin; Konstantinos T. Konstantinidis; Ulas Tezel;

  • 作者单位

    Institute of Environmental Sciences and;

    Center for Life Sciences and Technologies, Bogazici University, Bebek 34342 Istanbul, Turkey,School of Civil and Environmental Engineering and;

    Institute of Environmental Sciences and ,Center for Life Sciences and Technologies, Bogazici University, Bebek 34342 Istanbul, Turkey;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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