...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Long-Term Nickel Contamination Increases the Occurrence of Antibiotic Resistance Genes in Agricultural Soils
【24h】

Long-Term Nickel Contamination Increases the Occurrence of Antibiotic Resistance Genes in Agricultural Soils

机译：长期镍污染增加了农业土壤中抗生素抗性基因的发生

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Heavy metal contamination is assumed to be a selection pressure on antibiotic resistance, but to our knowledge, evidence of the heavy metal-induced changes of antibiotic resistance is lacking on a long-term basis. Using quantitative PCR array and Illumina sequencing, we investigated the changes of a wide spectrum of soil antibiotic resistance genes (ARGs) following 4-5 year nickel exposure (0-800 mg kg~(-1)) in two long-term experimental sites. A total of 149 unique ARGs were detected, with multidrug and β-lactam resistance as the most prevailing ARG types. The frequencies and abundance of ARGs tended to increase along the gradient of increasing nickel concentrations, with the highest values recorded in the treatments amended with 400 mg nickel kg~(-1) soiL The abundance of mobile genetic elements (MGEs) was significantly associated with ARGs, suggesting that nickel exposure might enhance the potential for horizontal transfer of ARGs. Network analysis demonstrated significant associations between ARGs and MGEs, with the integrase intI1 gene having the most frequent interactions with other co-occurring ARGs. The changes of ARGs were mainly driven by nickel bioavailability and MGEs as revealed by structural equation models. Taken together, long-term nickel exposure significantly increased the diversity, abundance, and horizontal transfer potential of soil ARGs.
机译：重金属污染被认为是对抗生素耐药性的选择压力，但据我们所知，长期缺乏重金属诱导的抗生素耐药性变化的证据。使用定量PCR阵列和Illumina测序，我们在两个长期实验地点研究了4-5年镍暴露（0-800 mg kg〜（-1））后土壤抗生素抗性基因（ARG）的广泛变化。总共检测到149种独特的ARG，其中最常见的ARG类型是多药耐药和β-内酰胺耐药。 ARGs的频率和丰度倾向于随着镍浓度的增加而增加，其中以400 mg镍kg〜（-1）溶液修正的处理中记录的最高值。移动遗传元素（MGEs）的丰度与ARGs，表明镍暴露可能会增强ARGs水平转移的潜力。网络分析表明ARG和MGE之间存在显着关联，整合酶intI1基因与其他同时出现的ARG相互作用最频繁。结构方程模型揭示了ARGs的变化主要受镍生物利用度和MGE的驱动。两者合计，长期暴露于镍显着增加了土壤ARGs的多样性，丰度和水平转移潜力。

著录项

  • 来源
    《Environmental Science & Technology》 |2017年第2期|790-800|共11页
  • 作者

    Hang-Wei Hu; Jun-Tao Wang; Jing Li; Xiu-Zhen Shi; Yi-Bing Ma; Deli Chen; Ji-Zheng He;

  • 作者单位

    State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China Faculty,Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia;

    State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China Faculty;

    State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China Faculty;

    Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia;

    National Soil Fertility and Fertilizer Effects Long-term Monitoring Network, Institute of Agricultural Resources and Regional lanning, Chinese Academy of Agricultural Sciences, Beijing 100081, China;

    Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia;

    State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China Faculty,Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号