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首页> 外文期刊>Environmental Science & Technology >Temporal Dynamics of In-Field Bioreactor Populations Reflect the Groundwater System and Respond Predictably to Perturbation
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Temporal Dynamics of In-Field Bioreactor Populations Reflect the Groundwater System and Respond Predictably to Perturbation

机译:现场生物反应器种群的时间动态反映了地下水系统,并对扰动做出了可预测的响应

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摘要

Temporal variability complicates testing the influences of environmental variability on microbial community structure and thus function. An in-field bioreactor system was developed to assess oxic versus anoxic manipulations on in situ groundwater communities. Each sample was sequenced (16S SSU rRNA genes, average 10,000 reads), and biogeochemical parameters are monitored by quantifying 53 metals, 12 organic acids, 14 anions, and 3 sugars. Changes in dissolved oxygen (DO), pH, and other variables were similar across bioreactors. Sequencing revealed a complex community that fluctuated in-step with the groundwater community and responded to DO. This also directly influenced the pH, and so the biotic impacts of DO and pH shifts are correlated. A null model demonstrated that bioreactor communities were driven in part not only by experimental conditions but also by stochastic variability and did not accurately capture alterations in diversity during perturbations. We identified two groups of abundant OTUs important to this system; one was abundant in high DO and pH and contained heterotrophs and oxidizers of iron, nitrite, and ammonium, whereas the other was abundant in low DO with the capability to reduce nitrate. In-field bioreactors are a powerful tool for capturing natural microbial community responses to alterations in geochemical factors beyond the bulk phase.
机译:时间变异性使测试环境变异性对微生物群落结构及其功能的影响变得复杂。开发了一个现场生物反应器系统,以评估原位地下水群落的有氧与无氧操作。对每个样品进行测序(16S SSU rRNA基因,平均10,000个读数),并通过定量53种金属,12种有机酸,14种阴离子和3种糖来监测生物地球化学参数。跨生物反应器的溶解氧(DO),pH和其他变量的变化相似。测序揭示了一个复杂的社区,该社区与地下水社区同步波动并响应DO。这也直接影响了pH值,因此DO和pH值变化对生物的影响是相关的。无效模型表明,生物反应器群落不仅部分受到实验条件的驱动,还受到随机变异性的驱动,并且不能准确地捕获扰动过程中多样性的变化。我们确定了两组对这个系统很重要的丰富OTU。一种富含高溶解氧和pH,并含有铁,亚硝酸盐和铵的异养生物和氧化剂,而另一种富含低溶解氧,具有还原硝酸盐的能力。现场生物反应器是一种强大的工具,可捕获天然微生物群落对超出本体相的地球化学因子变化的反应。

著录项

  • 来源
    《Environmental Science & Technology》 |2017年第5期|2879-2889|共11页
  • 作者单位

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States;

    Department of Environmental Health and Enginering, Johns Hopkins University, Baltimore, Maryland 21218, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States;

    Fish, Wildlife and Conservation Biology, Colorado State University, Fort Collins, Colorado 80523, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States ,Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States ,Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States ,Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States;

    Department of Microbiology and Plant Biology, University of Oklahoma, Norman, Oklahoma 73019, United States;

    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, United States;

    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States;

    Department of Microbiology & Immunology, Montana State University, Bozeman, Montana 59717, United States;

    Environmental Genomics and Systems Biology Division, Lawrence Berkley National Laboratory, Berkley, California 94720, United States;

    Civil and Environmental Engineering and Biological Engineering, Massachusets Institute of Technology, Cambridge, Massachusetts 02139, United States;

    Department of Microbiology and Plant Biology, University of Oklahoma, Norman, Oklahoma 73019, United States;

    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States ,Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States;

    Environmental Genomics and Systems Biology Division, Lawrence Berkley National Laboratory, Berkley, California 94720, United States;

    Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008, MS-6036, Oak Ridge, Tennessee 37831-6036, United States ,Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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