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首页> 外文期刊>Environmental Science & Technology >Comparison of Metals and Tetracycline as Selective Agents for Development of Tetracycline Resistant Bacterial Communities in Agricultural Soil
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Comparison of Metals and Tetracycline as Selective Agents for Development of Tetracycline Resistant Bacterial Communities in Agricultural Soil

机译：金属和四环素作为选择剂在农业土壤中发展抗四环素细菌群落的比较

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摘要

Environmental selection of antibiotic resistance may be caused by either antibiotic residues or coselecting agents. Using a strictly controlled experimental design, we compared the ability of metals (Cu or Zn) and tetracycline to (co)select for tetracycline resistance in bacterial communities. Soil microcosms were established by amending agricultural soil with known levels of Cu, Zn, or tetracycline known to represent commonly used metals and antibiotics for pig farming. Soil bacterial growth dynamics and bacterial community-level tetracycline resistance were determined using the [~3H]leucine incorporation technique, whereas soil Cu, Zn, and tetracycline exposure were quantified by a panel of whole-cell bacterial bioreporters. Tetracycline resistance increased significantly in soils containing environmentally relevant levels of Cu (≥365 mg kg~(-1)) and Zn (≥264 mg kg~(-1)) but not in soil spiked with unrealistically high levels of tetracycline (up to 100 mg kg~(-1)). These observations were consistent with bioreporter data showing that metals remained bioavailable, whereas tetracycline was only transiently bioavailable. Community-level tetracycline resistance was correlated to the initial toxicant-induced inhibition of bacterial growth. In conclusion, our study demonstrates that toxic metals in some cases may exert a stronger selection pressure for environmental selection of resistance to an antibiotic than the specific antibiotic itself.
机译：抗生素耐药性的环境选择可能由抗生素残留物或共选择剂引起。使用严格控制的实验设计，我们比较了细菌群落中金属（铜或锌）和四环素与（共）选择四环素抗性的能力。土壤微观世界是通过用已知水平的铜，锌或四环素修正农业土壤来建立的，已知铜，锌或四环素可代表用于养猪业的常用金属和抗生素。使用[〜3H]亮氨酸掺入技术确定土壤细菌的生长动力学和细菌群落水平的四环素抗性，而土壤铜，锌和四环素的暴露量则通过一组全细胞细菌生物报告进行定量。在含有与环境有关的铜（≥365mg kg〜（-1））和锌（≥264mg kg〜（-1））的土壤中，四环素抗性显着提高，但在掺入了不切实际的高四环素（达到最高水平）的土壤中，四环素抗性却没有明显提高。 100 mg kg〜（-1））。这些观察结果与生物报告者的数据一致，后者显示金属仍然具有生物利用度，而四环素仅具有瞬时生物利用度。社区水平的四环素抗性与初始有毒物诱导的细菌生长抑制有关。总而言之，我们的研究表明，在某些情况下，有毒金属可能比特定抗生素本身对环境抗药性施加更大的选择压力。

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  • 来源
    《Environmental Science & Technology》 |2017年第5期|3040-3047|共8页
  • 作者

    Jianxiao Song; Christopher Rensing; Peter E. Holm; Marko Virta; Kristian K Brandt;

  • 作者单位

    Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg, Denmark;

    College of Natural Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;

    Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg, Denmark;

    Department of Food and Environmental Sciences, University of Helsinki, PL 56 Helsinki, Finland;

    Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg, Denmark;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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