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Variation of Bacterial Communities with Water Quality in an Urban Tropical Catchment

机译：城市热带流域细菌群落的水质变化

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摘要

A major challenge for assessment of water quality in tropical environments is the natural occurrence and potential growth of Fecal Indicator Bacteria (FIB). To gain a better understanding of the relationship between measured levels of FIB and the distribution of sewage-associated bacteria, including potential pathogens, in the tropics this study compared the abundance of FIB (Total coliforms and E. coli) and the Bacteroidales (HF183 marker) with bacterial community structure determined by next-generation amplicon sequencing. Water was sampled twice over 6 months from 18 sites within a tropical urban catchment and reservoir, followed by extraction of DNA from microorganisms, and sequencing targeting the V3-V4 region of the 16S rRNA gene. Multivariate statistical analyses indicated that bacterial community composition (BCC) varied between reservoir and catchment, within catchment land.uses, and with E. coli concentration. Beta-regression indicated that the proportion of sequences from sewage-associated taxa (SAT) or pathogen-like sequences (PLS) were predicted most significantly by measured levels of E. coli(log MPN/100 mL) (χ~2 > 8.7； ρ < 0.003). In addition, SAT were significantly predicted by log HF183 levels (χ~2=13.1; ρ = 0.0003) while PLS were not Our study suggests that measurements of E. coli concentration could be useful in predicting samples enriched in sewage-associated and pathogen-like bacteria in tropical environments despite the potential for nonconservative behavior.
机译：评估热带环境中水质的主要挑战是粪便指示细菌（FIB）的自然发生和潜在生长。为了更好地了解FIB的测定水平与热带地区与污水相关细菌（包括潜在病原体）的分布之间的关系，本研究比较了FIB（总大肠菌群和大肠杆菌）和拟杆菌（HF183标记）的丰度）具有通过下一代扩增子测序确定的细菌群落结构。在六个月内，从一个热带城市集水区和水库内的18个地点采样了两次水，然后从微生物中提取DNA，并针对16S rRNA基因的V3-V4区进行测序。多变量统计分析表明，水库和集水区之间，集水区土地用途和大肠杆菌浓度之间细菌群落组成（BCC）有所不同。 Beta回归表明，通过测定大肠杆菌水平（log MPN / 100 mL），最能预测污水相关分类群（SAT）或类病原体序列（PLS）序列的比例（χ〜2> 8.7； ρ<0.003）。此外，SAT可以通过对数HF183水平显着预测（χ〜2 = 13.1;ρ= 0.0003），而PLS则不然。我们的研究表明，大肠杆菌浓度的测量可用于预测富含污水相关和病原体的样品。像热带环境中的细菌一样，尽管可能具有非保守行为。

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  • 来源
    《Environmental Science & Technology》 |2017年第10期|5591-5601|共11页
  • 作者

    Jean Pierre Nshimyimana; )Adam Joshua Ehrich Freedman; Peter Shanahan; Lloyd C. H. Chua; Janelle R. Thompson;

  • 作者单位

    School of Civil and Environmental Engineering, Nanyang Technological University (NTU), 50 Nanyang Avenue, Singapore 639798, Singapore,Department of Civil and Environmental Engineering, Massachusetts Institute of Technology (MIT), 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States,Centre for Environmental Sensing and Modeling (CENSAM), Singapore-MIT Alliance for Research and Technology (SMART), 1 Create Way, Singapore 138602, Singapore,Singapore Center on Environmental Life Sciences Engineering (SCELSE), NTU, 60 Nanyang Drive, Singapore 637551, Singapore;

    Department of Civil and Environmental Engineering, Massachusetts Institute of Technology (MIT), 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States,Centre for Environmental Sensing and Modeling (CENSAM), Singapore-MIT Alliance for Research and Technology (SMART), 1 Create Way, Singapore 138602, Singapore;

    Department of Civil and Environmental Engineering, Massachusetts Institute of Technology (MIT), 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States,Centre for Environmental Sensing and Modeling (CENSAM), Singapore-MIT Alliance for Research and Technology (SMART), 1 Create Way, Singapore 138602, Singapore;

    School of Engineering, Deakin University, Waum Ponds, Geelong, Victoria 3216, Australia;

    Department of Civil and Environmental Engineering, Massachusetts Institute of Technology (MIT), 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States,Centre for Environmental Sensing and Modeling (CENSAM), Singapore-MIT Alliance for Research and Technology (SMART), 1 Create Way, Singapore 138602, Singapore;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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