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首页> 外文期刊>Environmental Science & Technology >Heterologous Expression of Pteris vittata Arsenite Antiporter PvACR3;1 Reduces Arsenic Accumulation in Plant Shoots
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Heterologous Expression of Pteris vittata Arsenite Antiporter PvACR3;1 Reduces Arsenic Accumulation in Plant Shoots

机译:蕨类植物亚砷逆向转运蛋白PvACR3; 1的异源表达减少植物芽中砷的积累

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摘要

Arsenic (As) is a toxic carcinogen so it is crucial to decrease As accumulation in crops to reduce its risk to human health. Arsenite (AsIII) antiporter ACR3 protein is critical for As metabolism in organisms, but it is lost in flowering plants. Here, a novel ACR3 gene from As- hyperaccumulator Pteris vittata, PvACR3;1, was cloned and expressed in Saccharomyces cerevisiae (yeast), Arabidopsis thaliana (model plant), and Nicotiana tabacum (tobacco). Yeast experiments showed that PvACR3;1 functioned as an AsIII-antiporter to mediate AsIII efflux to an external medium. At 5 μM AsIII, PvACR3;1 transgenic Arabidopsis accumulated 14- 29% higher As in the roots and 55-61% lower As in the shoots compared to WT control, showing lower As translocation. Besides, transgenic tobacco under 5 μM AsIII or AsV also showed similar results, indicating that expressing PvACR3;1 gene increased As retention in plant roots. Moreover, observation of PvACR3;1-green fluorescent protein fusions in transgenic Arabidopsis showed that PvACR3;1 protein localized to the vacuolar membrane, indicating that PvACR3;1 mediated AsIII sequestration into vacuoles, consistent with increased root As. In addition, soil experiments showed ~22% lower As in the shoots of transgenic tobacco than control. Thus, our study provides a potential strategy to limit As accumulation in plant shoots, representing the first report to decrease As translocation by sequestrating AsIII into vacuoles, shedding light on engineering low-As crops to improve food safety.
机译:砷(As)是一种有毒的致癌物,因此降低砷在作物中的积累以降低其对人体健康的风险至关重要。砷(AsIII)反向转运蛋白ACR3蛋白对于有机体中的砷代谢至关重要,但在开花植物中会丢失。在此,克隆了来自As-超蓄积性翼龙的新ACR3基因PvACR3; 1,并在酿酒酵母(酵母),拟南芥(模型植物)和烟草(烟草)中表达。酵母实验显示PvACR3; 1充当AsIII反向转运蛋白,介导AsIII外排。与WT对照相比,在5μMAsIII下,PvACR3; 1转基因拟南芥在根中的As积累高14-29%,在芽中的As降低55-61%,显示出较低的As易位。此外,在5μMAsIII或AsV下转基因烟草也显示出相似的结果,表明表达PvACR3; 1基因可增加植物根中的As保留量。此外,在转基因拟南芥中观察到PvACR3; 1-绿色荧光蛋白融合物显示PvACR3; 1蛋白位于液泡膜上,表明PvACR3; 1介导的AsIII螯合进入液泡,与增加的根As一致。此外,土壤实验表明,转基因烟草芽中的砷比对照低约22%。因此,我们的研究提供了一种潜在的策略来限制植物芽中As的积累,这是首次报道通过将AsIII螯合到液泡中,减少工程化低砷作物以提高食品安全性来减少As易位的报道。

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  • 来源
    《Environmental Science & Technology》 |2017年第18期|10387-10395|共9页
  • 作者单位

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China,Quangang Petrochemical Research Institute, Fujian Normal University, Quanzhou, Fujian 326801, China;

    Research Institute of Rural Sewage Treatment, South West Forestry University, Yunnan 650224, China;

    Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, United States;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China,Soil and Water Science Department, University of Florida, Gainesville, Florida 32611, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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