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首页> 外文期刊>Environmental Science & Technology >Enzymatic Purification of Microplastics in Environmental Samples
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Enzymatic Purification of Microplastics in Environmental Samples

机译：酶法纯化环境样品中的微塑料

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摘要

Micro-Fourier transform infrared (micro-FTIR) spectroscopy and Raman spectroscopy enable the reliable identification and quantification of microplastics (MPs) in the lower micron range. Since concentrations of MPs in the environment are usually low, the large sample volumes required for these techniques lead to an excess of coenriched organic or inorganic materials. While inorganic materials can be separated from MPs Wing density separation, the organic fraction impedes the ability to conduct reliable analyses. Hence, the purification of MPs from organic materials is crucial prior to conducting an identification via spectroscopic techniques. Strong, acidic or alkaline treatments bear the danger of degrading sensitive synthetic polymers. We suggest an alternative method, which uses a series of technical grade enzymes for purifying MPs in environmental samples. A basic enzymatic purification protocol (BEPP) proved to be efficient while reducing 98.3 +/- 0.1% of the sample matrix in surface water samples. After showing a high recovery rate (84.5 +/- 3.3%), the BEPP was successfully applied to environmental samples from the North Sea where numbers of MPs range from 0.05 to 4.42 items m(-3). Experiences with different environmental sample matrices were considered in an improved and universally applicable version of the BEPP, which is suitable for focal plane array detector (FPA)-based micro-FTIR analyses of water, wastewater, sediment, biota, and food samples.
机译：微傅里叶变换红外（micro-FTIR）光谱和拉曼光谱可实现对较低微米范围内的微塑料（MPs）的可靠鉴定和定量。由于环境中MP的浓度通常较低，因此这些技术所需的大量样品会导致过量富集的有机或无机材料。尽管可以从MP机翼密度分离中分离出无机材料，但有机物组分却阻碍了进行可靠分析的能力。因此，在通过光谱技术进行鉴定之前，从有机材料中纯化MP至关重要。强力，酸性或碱性处理具有降解敏感合成聚合物的危险。我们建议一种替代方法，该方法使用一系列工业级酶来纯化环境样品中的MP。基本的酶纯化方案（BEPP）被证明是有效的，同时减少了地表水样品中98.3 +/- 0.1％的样品基质。在显示出较高的回收率（84.5 +/- 3.3％）之后，BEPP成功地应用于北海的MP范围为0.05至4.42项m（-3）的环境样品。 BEPP的改进版本和通用版本考虑了不同环境样品基质的经验，该版本适用于基于焦平面阵列检测器（FPA）的水，废水，沉积物，生物群和食品样品的微型FTIR分析。

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  • 来源
    《Environmental Science & Technology》 |2017年第24期|14283-14292|共10页
  • 作者

    Loeder Martin G. J.; Imhof Hannes K.; Ladehoff Maike; Loeschel Lena A.; Lorenz Claudia; Mintenig Svenja; Piehl Sarah; Primpke Sebastian; Schrank Isabella; Laforsch Christian; Gerdts Gunnar;

  • 作者单位

    Helmholtz Zentrum Polar & Meeresforsch, Alfred Wegener Inst, Biol Anstalt Helgoland, POB 180, D-27483 Helgoland, Germany;

    Univ Bayreuth, Dept Anim Ecol 1, Univ Str 30, D-95440 Bayreuth, Germany;

    Helmholtz Zentrum Polar & Meeresforsch, Alfred Wegener Inst, Biol Anstalt Helgoland, POB 180, D-27483 Helgoland, Germany;

    Univ Bayreuth, Dept Anim Ecol 1, Univ Str 30, D-95440 Bayreuth, Germany;

    Helmholtz Zentrum Polar & Meeresforsch, Alfred Wegener Inst, Biol Anstalt Helgoland, POB 180, D-27483 Helgoland, Germany;

    Helmholtz Zentrum Polar & Meeresforsch, Alfred Wegener Inst, Biol Anstalt Helgoland, POB 180, D-27483 Helgoland, Germany;

    Univ Bayreuth, Dept Anim Ecol 1, Univ Str 30, D-95440 Bayreuth, Germany;

    Helmholtz Zentrum Polar & Meeresforsch, Alfred Wegener Inst, Biol Anstalt Helgoland, POB 180, D-27483 Helgoland, Germany;

    Univ Bayreuth, Dept Anim Ecol 1, Univ Str 30, D-95440 Bayreuth, Germany;

    Univ Bayreuth, Dept Anim Ecol 1, Univ Str 30, D-95440 Bayreuth, Germany;

    Helmholtz Zentrum Polar & Meeresforsch, Alfred Wegener Inst, Biol Anstalt Helgoland, POB 180, D-27483 Helgoland, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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