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首页> 外文期刊>Environmental Science & Technology >Ovatoxin-a, A Palytoxin Analogue Isolated from Ostreopsis cf. ovata Fukuyo: Cytotoxic Activity and ELISA Detection
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Ovatoxin-a, A Palytoxin Analogue Isolated from Ostreopsis cf. ovata Fukuyo: Cytotoxic Activity and ELISA Detection

机译：卵毒素-a，从骨质疏松症中分离出的一种palytoxin类似物。 ovata Fukuyo：细胞毒活性和ELISA检测

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摘要

This study provides the first evaluation of the cytotoxic effects of the recently identified palytoxin (PLTX) analog, ovatoxin-a (OVTX-a), the major toxin produced by Ostreopsis cf. ovata in the Mediterranean Sea. Its increasing detection during Ostreopsis blooms and in seafood highlights the need to characterize it$ toxic effects and to set up appropriate detection methods. OVTX-a is about 100 fold less potent than PLTX in reducing HaCaT cells viability (EC_(50) = 1.1 × 10~(-9) M vs 1.8 × 10~(-11) M, MTT test) in agreement with a reduced binding affinity (K_d = 1.2 × 10~(-9) vs 2.7 × 10~(-11) M, saturation experiments on intact cells). Similarly, OVTX-a hemolytic effect is lower than that of the reference PLTX compound. Ost- D shows the lowest cytotoxicity toward HaCaT keratinocytes, suggesting the lack of a hydroxyl group at C44 as a critical feature for PLTXs cytotoxic effects. A sandwich ELISA developed for PLTX detects also OVTX-a in a sensitive (LOD = 4.2 and LOQ= 5.6 ng/mL) and accurate manner (Bias = 0.3%), also in O. cf. ovata extracts and contaminated mussels. Although in vitro OVTX- a appears less toxic than PLTX, its cytotoxicity at nanomolar concentrations after short exposure time rises some concern for human health. The sandwich ELISA can be a viable screening method for OVTXs detection in monitoring program.
机译：这项研究首次评估了最近鉴定出的毒素（PLTX）类似物卵毒素-a（OVTX-a）的细胞毒性作用，卵毒素是由Ostreopsis cf.生产的主要毒素。卵在地中海。它在Ostreopsis花朵和海鲜中的检出率不断提高，这凸显了表征其毒性作用和建立适当检出方法的需要。 OVTX-a在降低HaCaT细胞活力方面的功效比PLTX低约100倍（EC_（50）= 1.1×10〜（-9）M vs 1.8×10〜（-11）M，MTT测试）结合亲和力（K_d = 1.2×10〜（-9）vs 2.7×10〜（-11）M，对完整细胞的饱和实验）。同样，OVTX-a的溶血作用低于参考PLTX化合物的溶血作用。 Ost-D对HaCaT角质形成细胞的毒性最低，表明C44缺少羟基是PLTXs细胞毒性作用的关键特征。为PLTX开发的夹心ELISA还以灵敏（LOD = 4.2和LOQ = 5.6 ng / mL）和准确方式（Bias = 0.3％）检测OVTX-a，同样以O.cf.为例。卵子提取物和受污染的贻贝。尽管体外OVTX-a的毒性不及PLTX，但短时间暴露后其纳摩尔浓度的细胞毒性引起了人们对健康的关注。夹心ELISA是监测程序中OVTX检测的可行筛选方法。

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  • 来源
    《Environmental Science & Technology》 |2016年第3期|1544-1551|共8页
  • 作者

    Marco Pelin; Martino Forino; Valentina Brovedani; Luciana Tartaglione; Carmela DellAversano; Rossella Pistocchi; Mark Poli; Silvio Sosa; Chiara Florio; Patrizia Ciminiello; Aurelia Tubaro;

  • 作者单位

    Department of Life Science, University of Trieste, 34127 Trieste, Italy;

    Department of Pharmacy, University of Napoli Federico Ⅱ, 80131 Napoli, Italy;

    Department of Life Science, University of Trieste, 34127 Trieste, Italy;

    Department of Pharmacy, University of Napoli Federico Ⅱ, 80131 Napoli, Italy;

    Department of Pharmacy, University of Napoli Federico Ⅱ, 80131 Napoli, Italy;

    Interdepartmental Center for Research in Environmental Sciences, University of Bologna, 481230 Ravenna, Italy;

    U.S. Army Medical Research Institute of Infectious Diseases, Ft. Detrick, Maryland, 21701-5011 United States;

    Department of Life Science, University of Trieste, 34127 Trieste, Italy;

    Department of Life Science, University of Trieste, 34127 Trieste, Italy;

    Department of Pharmacy, University of Napoli Federico Ⅱ, 80131 Napoli, Italy;

    Department of Life Sciences, University of Trieste, Via A. Valerio 6, 34127 Trieste, Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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