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首页> 外文期刊>Environmental Science & Technology >Hydrogen Isotope Fractionation As a Tool to Identify Aerobic and Anaerobic PAH Biodegradation
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Hydrogen Isotope Fractionation As a Tool to Identify Aerobic and Anaerobic PAH Biodegradation

机译：氢同位素分馏作为鉴定有氧和厌氧PAH生物降解的工具

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摘要

Aerobic and anaerobic polycyclic aromatic hydrocarbon (PAH) biodegradation was characterized by compound specific stable isotope analysis (CSIA) of the carbon and hydrogen isotope effects of the enzymatic reactions initiating specific degradation pathways, using naphthalene and 2-methylnaphtalene as model compounds. Aerobic activation of naphthalene and 2-methylnaphthalene by Pseudomonas putida NCIB 9816 and Pseudomonas fluorescens ATCC 17483 containing naphthalene dioxygenases was associated with moderate carbon isotope fractionation (ε_C = -0.8 ± 0.1‰ to -1.6 ± 0.2‰). In contrast, anaerobic activation of naphthalene by a carboxylation-like mechanism by strain NaphS6 was linked to negligible carbon isotope fractionation (ε_C = -0.2 ± 0.2‰ to -0.4 ± 0.3‰). Notably, anaerobic activation of naphthalene by strain NaphS6 exhibited a normal hydrogen isotope fractionation (ε_H = -11 ± 2‰ to -47 ± 4‰), whereas an inverse hydrogen isotope fractionation was observed for the aerobic strains (ε_H = +15 ± 2‰ to +71 ± 6‰). Additionally, isotope fractionation of NaphS6 was determined in an overlaying hydrophobic carrier phase, resulting in more reliable enrichment factors compared to immobilizing the PAHs on the bottle walls without carrier phase. The observed differences especially in hydrogen fractionation might be used to differentiate between aerobic and anaerobic naphthalene and 2-methylnaphthalene biodegradation pathways at PAH-contaminated field sites.
机译：需氧和厌氧多环芳烃（PAH）的生物降解的特征在于，使用萘和2-甲基萘作为模型化合物，通过酶促反应的碳和氢同位素效应启动特定的降解途径，通过化合物特异性稳定同位素分析（CSIA）。恶臭假单胞菌NCIB 9816和荧光假单胞菌ATCC 17483含有萘二加氧酶对萘和2-甲基萘的需氧活化与中等碳同位素分馏有关（ε_C= -0.8±0.1‰至-1.6±0.2‰）。相反，菌株NaphS6通过类似羧化作用的机制将萘的厌氧活化与可忽略不计的碳同位素分馏有关（ε_C= -0.2±0.2‰至-0.4±0.3‰）。值得注意的是，菌株NaphS6对萘的厌氧活化表现出正常的氢同位素分馏（ε_H= -11±2‰至-47±4‰），而好氧菌株的氢同位素分馏呈逆向变化（ε_H= +15±2 ‰至+71±6‰）。此外，在覆盖的疏水性载体相中测定了NaphS6的同位素分馏，与将PAHs固定在无载体相的瓶壁上相比，可得到更可靠的富集因子。观察到的差异（尤其是氢分馏中的差异）可用于区分有氧和无氧萘以及在受PAH污染的现场的2-甲基萘生物降解途径。

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  • 来源
    《Environmental Science & Technology》 |2016年第6期|3091-3100|共10页
  • 作者

    Steffen Kuemmel; Robert Starke; Gao Chen; Florin Musat; Hans H. Richnow; Carsten Vogt;

  • 作者单位

    UFZ-Helmholtz Centre for Environmental Research, Department of Isotope Biogeochemistry, Permoserstrasse 15, 04318 Leipzig, Germany,University of Freiburg, Faculty of Biology, Schaenzlestrasse 1, 79104 Freiburg, Germany;

    UFZ-Helmholtz Centre for Environmental Research, Department of Isotope Biogeochemistry, Permoserstrasse 15, 04318 Leipzig, Germany;

    MPI-Max Planck Institute for Marine Microbiology, Department of Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany;

    MPI-Max Planck Institute for Marine Microbiology, Department of Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany,UFZ-Helmholtz Centre for Environmental Research, Department of Isotope Biogeochemistry, Permoserstrasse 15, 04318 Leipzig, Germany;

    UFZ-Helmholtz Centre for Environmental Research, Department of Isotope Biogeochemistry, Permoserstrasse 15, 04318 Leipzig, Germany;

    UFZ-Helmholtz Centre for Environmental Research, Department of Isotope Biogeochemistry, Permoserstrasse 15, 04318 Leipzig, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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