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首页> 外文期刊>Environmental Science & Technology >Bioaccumulation of Multiwall Carbon Nanotubes in Tetrahymena thermophila by Direct Feeding or Trophic Transfer
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Bioaccumulation of Multiwall Carbon Nanotubes in Tetrahymena thermophila by Direct Feeding or Trophic Transfer

机译:通过直接进食或营养转移在嗜热四膜虫中多壁碳纳米管的生物富集。

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摘要

Consumer goods contain multiwall carbon nanotubes (MWCNTs) that could be released during product life cycles into the environment, where their effects are uncertain. Here, we assessed MWCNT bioaccumulation in the protozoan Tetrahymena thermophila via trophic transfer from bacterial prey (Pseudomonas aeruginosa) versus direct uptake from growth media. The experiments were conducted using ~(14)C-labeIed MWCNT (~(14)C-MWCNT) doses at or below 1 mg/L, which proved subtoxic since there were no adverse effects on the growth of the test organisms. A novel contribution of this study was the demonstration of the ability to quantify MWCNT bioaccumulation at low (sub μg/kg) concentrations accomplished by employing accelerator mass spectrometry (AMS). After the treatments with MWCNTs at nominal concentrations of 0.01 mg/L and 1 mg/L, P. aeruginosa adsorbed considerable amounts of MWCNTs: (0.18 ± 0.04) μg/mg and (21.9 ± 4.2) μg/mg bacterial dry mass, respectively. At the administered MWCNT dose of 0.3 mg/L, T. thermophila accumulated up to (0.86 ± 0.3) μg/mg and (3.4 ± 1.1) μg/mg dry mass by trophic transfer and direct uptake, respectively. Although MWCNTs did not biomagnify in the microbial food chain, MWCNTs bioaccumulated in the protozoan populations regardless of the feeding regime, which could make MWCNTs bioavailable for organisms at higher trophic levels.
机译:消费品包含多壁碳纳米管(MWCNT),这些碳纳米管可以在产品生命周期内释放到不确定其影响的环境中。在这里,我们通过营养从细菌猎物(铜绿假单胞菌)的营养转移评估了原生动物四膜虫嗜热菌中的MWCNT生物蓄积与从生长培养基中直接摄取相比。使用〜(14)C标记的MWCNT(〜(14)C-MWCNT)剂量等于或低于1 mg / L进行了实验,由于对测试生物的生长没有不利影响,因此证明是亚毒性的。这项研究的新贡献是通过使用加速器质谱(AMS)证明了在低浓度(subμg/ kg)下量化MWCNT生物蓄积的能力。用标称浓度为0.01 mg / L和1 mg / L的MWCNT处理后,铜绿假单胞菌吸附了大量的MWCNT:分别为(0.18±0.04)μg/ mg和(21.9±4.2)μg/ mg细菌干重。 。在施用的MWCNT剂量为0.3 mg / L时,嗜热衣原体通过营养转移和直接吸收分别积累至干质量的(0.86±0.3)μg/ mg和(3.4±1.1)μg/ mg。尽管MWCNT在微生物食物链中没有生物放大作用,但是MWCNT在原生动物种群中生物富集,而与饲喂方式无关,这可能使MWCNT在营养级别较高的生物上具有生物利用性。

著录项

  • 来源
    《Environmental Science & Technology》 |2016年第16期|8876-8885|共10页
  • 作者单位

    Bren School of Environmental Science and Management, Earth Research Institute and University of California Center for the Environmental Implications of Nanotechnology (UC CEIN), University of California, Santa Barbara, California 93106, United States,Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, Tallinn 12618, Estonia;

    Biosystems and Biomaterials Division, Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States;

    Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory, Livermore, California 94550, United States;

    Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, California 93106, United States;

    Bren School of Environmental Science and Management, Earth Research Institute and University of California Center for the Environmental Implications of Nanotechnology (UC CEIN), University of California, Santa Barbara, California 93106, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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