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首页> 外文期刊>Environmental Science & Technology >Cell Surface Display Fungal Laccase as a Renewable Biocatalyst for Degradation of Persistent Micropollutants Bisphenol A and Sulfamethoxazole
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Cell Surface Display Fungal Laccase as a Renewable Biocatalyst for Degradation of Persistent Micropollutants Bisphenol A and Sulfamethoxazole

机译：细胞表面展示真菌漆酶作为可降解持久性微污染物双酚A和磺胺甲恶唑的可再生生物催化剂

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摘要

Fungal laccases have high activity in degrading various persistent organic pollutants. However, using enzymes in solution for water treatment has limitations of nonreusability, short enzyme lifetimes, and high cost of single use. In this study, we developed a new type of biocatalyst by immobilizing fungal laccase on the surface of yeast cells using synthetic biology techniques. The biocatalyst, referred to as surface display laccase (SDL), had an enzyme activity of 104 ± 3 mU/g dry cell (with 2,2-azinobis-3- ethylbenzothiazoline-6-sulfonate (ABTS)). The SDL retained over 90% of the initial enzyme activity after 25 days storage at room temperature, while, in contrast, activity of free laccase declined to 60% of its initial activity. The SDL could be reused with high stability as it retained 74% of initial activity after eight repeated batch reactions. Proof-of-concept evaluations of the effectiveness of SDL in treating contaminants of emerging concern were performed with bisphenol A and sulfamethoxazole. Results from contaminant degradation kinetics and the effects of redox mediator amendment provided insights into the factors affecting the efficacy of the SDL system. This study reports, for the first time, the development of a surface display enzyme biocatalyst as an effective and renewable alternative for treating recalcitrant organic micropollutants.
机译：真菌漆酶在降解各种持久性有机污染物方面具有很高的活性。然而，在溶液中使用酶进行水处理具有不可重复使用，酶寿命短和单次使用成本高的局限性。在这项研究中，我们通过使用合成生物学技术将真菌漆酶固定在酵母细胞表面上，开发了一种新型的生物催化剂。该生物催化剂被称为表面展示漆酶（SDL），其酶活为104±3 mU / g干细胞（含2,2-叠氮基双-3-乙基苯并噻唑啉-6-磺酸盐（ABTS））。在室温下储存25天后，SDL保留了超过90％的初始酶活性，​​相反，游离漆酶的活性下降到其初始活性的60％。 SDL可以重复使用，具有很高的稳定性，因为它在八次重复分批反应后保留了74％的初始活性。使用双酚A和磺胺甲恶唑对SDL处理新兴污染物的有效性进行了概念验证评估。污染物降解动力学的结果以及氧化还原介体修正的影响为了解影响SDL系统功效的因素提供了见解。这项研究首次报道了表面展示酶生物催化剂的开发，该酶是一种有效且可再生的替代品，用于处理顽固的有机微污染物。

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  • 来源
    《Environmental Science & Technology》 |2016年第16期|8799-8808|共10页
  • 作者

    Yingying Chen; Brooke Stemple; Manish Kumar; Na Wei;

  • 作者单位

    Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, Indiana 46556, United States;

    Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, Indiana 46556, United States;

    Department of Chemical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, United States;

    Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, Indiana 46556, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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